Changes in Levels of Solutes During Osmotic Adjustment to Water Stress in Leaves of Four Tropical Pasture Species

Abstract

Three tropical grasses, green panic (Panicum maximum var. trichoglume), buffel grass (Cenchrus ciliaris), and spear grass (Heteropogon contortus), and a tropical legume, siratro (Macroptilium atropurpureum), were field-grown in a semi-arid environment. One set of plants was well watered, while another set was subjected to a continuous 35-day drying cycle. Samples of specific leaves were taken at the beginning, middle and end of the drying cycle, and 1, 5 and 14 days after rewatering.

The major low-molecular-weight solutes which accumulated in the grasses during water stress were the inorganic ions sodium (green panic), potassium (buffel and spear grass) and chloride (all grasses). Accumulation of these ions largely accounted for the osmotic adjustments determined from a previous study of water relations of the leaves. Concentrations of the minor constituents glucose and fructose increased only slightly in the stressed grasses, whereas levels of sucrose, the major carbohydrate component, increased substantially, particularly in spear grass. Inositol accumulated to a small extent in spear grass. Differences between the grasses were evident in the organic acid spectrum and also in changes in concentration of organic acids due to water stress. In water-stressed tissue, malate levels increased in green panic and spear grass but were reasonably constant in buffel grass. Aconitate concentrations (not detected in buffel grass) decreased in stressed green panic, but increased in spear grass. Oxalate (only trace quantities in spear grass) was a major component in green panic and buffel grass, but did not appear to vary with increase in water stress. Succinate accumulated only in stressed spear grass. The contribution of carbohydrates and organic acids to the osmotic adjustment was relatively small. Proline accumulated to varying degrees in all stressed grasses. Betaine occurred only in trace amounts in spear grass, but accumulated substantially in green panic and buffel grass.

Water-stressed leaves of siratro did not accumulate inorganic ions, sugars, organic acids, proline or betaine, but pinitol levels increased.

Implications of the results relating osmotic adjustment to changes in chemical composition are discussed.