Interaction of stomatal responses to ABA and CO₂ in Arabidopsis thaliana

Abstract

Stomatal responses to ABA and CO₂ were investigated in Arabidopsis thaliana (L.) Heynh. wild-type and ABA insensitive mutants (abi1-1, abi2-1, abi1-1abi2-1) at the whole plant and at the isolated epidermis levels. In wild-type plants, feeding roots with ABA (1–50 µM) triggered a rapid drop in leaf conductance which levelled off during the following photoperiods, and strongly inhibited the increase in conductance induced by light. The rapid response was strongly inhibited in abi1-1, abi2-1 and abi1-1abi2-1 double mutants, but a residual long-term decrease in leaf conductance was still observed. In wild-type plants, exogenous ABA strongly enhanced the response to CO₂ removal. Conversely, in the absence of CO₂ the effect of ABA was drastically reduced in epidermal strip experiments. These results reveal a strong interaction between sensing of ABA and CO₂ in stomata of A. thaliana. Despite an initially wide stomatal aperture in abi-1, abi-2 and double mutant plants, their stomatal responses to light and CO₂ removal were half those of wild-type plants. Moreover these responses were totally independent of the presence of ABA, suggesting that ABI1 and ABI2 are either directly involved in the interaction between the two signalling pathways or, alternatively located upstream of this point of interaction.