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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Ultrastructural Localization of Ions. II. X-Ray Analytical Verification of Silver Precipitation Products and Distribution of Chloride in Mesophyll Cells of Barley Seedlings

RFMV Steveninck, B Ballment, PD Peters and TA Hall

Australian Journal of Plant Physiology 3(3) 359 - 365
Published: 1976

Abstract

The ultrastructural localization of Cl- by means of a silver precipitation technique has been verified by means of X-ray analytical electron microscopy (EMMA-4 electron microscope plus KEVEX energy dispersive analyser). It was shown that the localization method was specific for Cl- in the case of electron-dense deposits in chloroplasts, plasmodesmata and plasmalemma of barley mesophyll cells.

It was pointed out that the use of nitric acid rinses in order to remove non-specific deposits of silver phosphates and carbonates has pitfalls and that the precipitation technique should always be used in conjunction with X-ray microprobe analysis.

https://doi.org/10.1071/PP9760359

© CSIRO 1976

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