Consequences of salt stress on conductance to CO₂ diffusion, Rubisco characteristics and anatomy of spinach leaves

Abstract

Spinach (Spinacia oleracea L.) leaves stressed by irrigation with water containing 1% (w/v) NaCl for 20 days had low conductance to CO₂ diffusion both at the stomata and in the mesophyll. Mesophyll anatomy changed in salt-stressed leaves, which could have accounted for the decreased mesophyll conductance. Ribulose- 1,5-bisphosphate carboxylase/oxygenase in vitro activity and content were not affected by up to 20 days exposure to salinity but decreased when leaves were exposed to salt stress for longer than 20 days. Salt accumulation also caused a drop of Ca and Mg which might have decreased membrane stability and chlorophyll content, respectively. Measurements of chlorophyll fluorescence indicated that the 20-day-long salt stress did not directly affect photochemistry. We conclude that salinity reduces photosynthesis primarily by reducing the diffusion of CO₂ to the chloroplast, both by stomatal closure and by changes in mesophyll structure which decreases the conductance to CO₂ diffusion within the leaf. The capacity for carbon metabolism is eventually reduced but that occurs after substantial decreases in the conductance to CO₂ diffusion.