Photosynthesis, carbon partitioning and metabolite content during drought stress in peach seedlings

Abstract

Photosynthesis, metabolic carbon partitioning and the contribution of sorbitol to the osmotic potential of mature peach (Prunus persica (L.) Batsch) leaves and phloem sap were examined in plants undergoing two levels of a short term drought stress. The relationship between osmotic potential at full turgor and water potential showed that neither mild nor severe drought stress induced a significant active osmotic adjustment in mature leaves. The osmotic potential of leaves at full turgor was -1.9 MPa; sorbitol was the major organic component (20%). Leaf sucrose and starch contents were significantly reduced by drought. The partitioning of newly-fixed carbon was also affected by stress. These changes appeared to originate from the inhibition of photosynthesis induced by drought stress. At low photosynthetic rates, the turnover of organic ions was low, and sorbitol synthesis was favoured over that of sucrose. Water stress did not affect the in vitro activity of sucrose phosphate synthase (EC 2.4.1.14), the key enzyme in sucrose synthesis. The in vitro activity of aldose-6-phosphate reductase (EC 1.1.1.200), the key enzyme in sorbitol synthesis, tended to increase linearly in response to drought stress. It is concluded that, contrary to some other polyol-synthesising species, peach did not seem to benefit from sorbitol synthesis during short term drought stress for active osmotic adjustment in mature leaves. However, in phloem sap, increases in sucrose and especially sorbitol concentration were observed in stressed plants.