The Role of Sucrose-Phosphate Synthase in the Control of Photosynthate Partitioning in Zea mays Leaves

Abstract

The influence of light and leaf sucrose content on partitioning of photosynthate and sucrose-phosphate synthase (SPS) activity in maize (Zea mays L.) leaves was investigated. The ratio of partitioning of photosynthate between sucrose and starch shifted from about 17:1 to 2:1 when the irradiance was increased from 180 to 1450 µmol quanta m^-2 s^-1. Increasing the sucrose content of the leaves had little effect on the partitioning ratio. SPS from illuminated leaves had a higher affinity for its substrates, UDPGlc and Fru6P, and was less inhibited by P_i than the enzyme from darkened leaves but the V_max was unaffected. SPS was fully light activated at an irradiance of 340 but not 180 µmol quanta m^-2 s^-1. Increasing the sucrose content of maize leaves more than 3-fold had little or no effect on the activation state of SPS which, together with the partitioning data, suggests that sucrose does not exert significant short-term feedback inhibition of its own synthesis in this species.