Biological nitrogen fixation associated with tropical pasture grasses

Abstract

The semi-humid or humid tropics are ideal for the production of large quantities of biomass from fast-growing C₄ grasses, but high yields normally require large quantities of fertiliser, especially N, which has a very high input from fossil fuels (natural gas). A program has been started recently to use elephant grass (Pennisetum purpureum Schum.) to substitute firewood as a fuel and also to make charcoal for iron production. In this case, any large N fertiliser additions would mean that the yield of bio fuel per unit of fossil fuel invested would be detrimentally affected. In this study, we report on the potential for the selection of genotypes of fast-growing C₄ tropical grasses of the genera Pennisetum and Brachiaria for their capacity to obtain N inputs from plant-associated biological nitrogen fixation (BNF). Fourteen genotypes each of Brachiaria and Pennisetum were screened for BNF contributions by growing them in ¹⁵N-labelled soil. In the case of the Pennisetum, after a suitable cutting height for the crop had been selected, there were large differences in dry matter production, N accumulation and ¹⁵N enrichment. The differences in ¹⁵N enrichment between genotypes were statistically significant and BNF inputs were estimated as high as 41% of accumulated N. In the study on Brachiaria genotypes, potential inputs of BNF seemed lower. Only one or two genotypes of B. brizantha and B. ruziziensis obtained more then 20% of their N from BNF. The N₂-fixing bacteria that were most commonly associated with shoots and roots the Pennisetum genotypes were of the genus Herbaspirillum, but predominantly of a recently described new species. The Brachiaria spp. from three different sites (Rio de Janeiro, Goânia, Bahia) were predominately colonised by Azospirillum spp., most of the isolates being of the species Azospirillum amazonense. Very few Herbaspirilla were isolated from these plants.