Overexpression of HvVDE gene improved light protection in transgenic tobacco (Nicotiana tabacum)

Xiaojie Qu; Deyu Che; Fangting Qin; Guang Huang; Hongzhang Liu

doi:10.1071/FP24180

RESEARCH ARTICLE (Open Access)

Next Contents Vol 51(11)

Overexpression of HvVDE gene improved light protection in transgenic tobacco (Nicotiana tabacum)

Xiaojie Qu

^A ^B , Deyu Che ^A ^B , Fangting Qin ^A , Guang Huang ^C and Hongzhang Liu ^B ^*

+ Author Affiliations

- Author Affiliations

^A College of Food and Pharmaceutical Engineering, Liubao Tea Modern Industry College, Wuzhou University, Guangxi, China.

^B College of Life Sciences, Jilin Agricultural University, Changchun, China.

^C Wuzhou Shengfeng Ecological Technology Development Co., Ltd, Guangxi, China.

^* Correspondence to: liuslab@126.com

Handling Editor: Suleyman Allakhverdiev

Functional Plant Biology 51, FP24180 https://doi.org/10.1071/FP24180

Submitted: 16 July 2024 Accepted: 11 October 2024 Published: 11 November 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Hosta is commonly acknowledged as a popular and preferred plant for landscaping and gardening. The ‘sunburn’ caused by prolonged exposure to strong sunlight is reducing the ornamental values of Hosta plants. However, there is a scarcity of research focusing on the genetic components linked to light-induced harm in Hosta. Here, the violaxanthin de-epoxidase (VDE) homolog from Hosta ventricosa was isolated and functionally identified through conducting HvVDE-overexpression tobacco (Nicotiana tabacum) lines. The results showed that HvVDE encodes a putative protein comprising 481 amino acids with a molecular weight of 54.304 kDa. The phylogenetic analysis found that HvVDE exhibited close similarity to JcVDE. Besides, the expression patterns of HvVDE found that HvVDE was expressed differently across tissues, withexpression induced by high light intensities. And overexpression of HvVDE led to the restoration of non-photochemical quenching in tobacco, suggesting that HvVDE plays a role in dissipating excess light energy as thermal energy in H. ventricosa. These findings underscore the significance of HvVDE in mitigating photoinhibition and enhancing photoprotection mechanisms in H. ventricosa.

Keywords: ectopic expression, H. ventricosa, HvVDE, light stress, molecular breeding, non-photochemical quenching, photoprotection, xanthophyll cycle.

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