Understanding the role of the fructose-1,6-bisphosphatase gene for enhancing the photosynthetic rate in Arabidopsis thaliana
Fatima Gulzar A , Raza Ahmad B , Suk-Yoon Kwan C , Zulqurnain Khan A , Sulaiman Ali Alharbi D , Mohmmad Maroof Shah B , Shoaib ur Rehman A , Maria Siddique E , Mohammad Javed Ansari F G , Irum Shahzadi B , Muhammad Abu Bakar Saddique A , Muhmmad Zahid Ishaq H and Ummara Waheed A *A
B
C
D
E
F
G
H
Abstract
Transgenic Arabidopsis thaliana (ecotype Columbia) was successfully transformed with the gene fructose-1,6-bisphosphatase (FBPase) and named as AtFBPase plants. Transgenic plants exhibited stable transformation, integration and significantly higher expressions for the transformed gene. Morphological evaluation of transgenic plants showed increased plant height (35 cm), number of leaves (25), chlorophyll contents (28%), water use efficiency (increased from 1.5 to 2.6 μmol CO2 μmol−1 H2O) and stomatal conductance (20%), which all resulted in an enhanced photosynthetic rate (2.7 μmol m−2 s−1) compared to wild type plants. This study suggests the vital role of FBPase gene in the modification of regulatory pathways to enhance the photosynthetic rate, which can also be utilised for economic crops in future.
Keywords: Arabidopsis thaliana, C3, C4, CO2, FBPase, genetic transformation, photorespiration, photosynthesis.
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