Physiological and transcriptome analysis of Dendrobium officinale under low nitrogen stress
Hangtao Wu A § , Jin Li A § , Qian Pu A , Chunyi Mi A , Guohong Zeng A , Ying Chen A , Dedong Kong B , Xiaorong Zuo C , Xiufang Hu
A * and Ou Li A *
+ Author Affiliations
- Author Affiliations
A Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, P.R. China.
B Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310018, P.R. China.
C Xi’an Ande Pharmaceutical Co., Ltd, Zhenping Branch, Xi’an 710000, P.R. China.
Functional Plant Biology 50(4) 314-334 https://doi.org/10.1071/FP22061
Submitted: 1 April 2022 Accepted: 6 February 2023 Published: 6 March 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Nitrogen (N) is the main nutrient of plants, and low nitrogen usually affects plant growth and crop yield. The traditional Chinese herbal medicine Dendrobium officinale Kimura et. Migo is a typical low nitrogen-tolerant plant, and its mechanism in response to low nitrogen stress has not previously been reported. In this study, physiological measurements and RNA-Seq analysis were used to analyse the physiological changes and molecular responses of D. officinale under different nitrogen concentrations. The results showed that under low nitrogen levels, the growth, photosynthesis and superoxide dismutase activity were found to be significantly inhibited, while the activities of peroxidase and catalase, the content of polysaccharides and flavonoids significantly increased. Differentially expressed genes (DEGs) analysis showed that nitrogen and carbon metabolisms, transcriptional regulation, antioxidative stress, secondary metabolite synthesis and signal transduction all made a big difference in low nitrogen stress. Therefore, copious polysaccharide accumulation, efficient assimilation and recycling of nitrogen, as well as rich antioxidant components play critical roles. This study is helpful for understanding the response mechanism of D. officinale to low nitrogen levels, which might provide good guidance for practical production of high quality D. officinale.
Keywords: abiotic stress, Dendrobium officinale, flavonoids, low nitrogen stress, physiological, polysaccharides, responses to nitrogen treatment, transcriptomic.
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