New insights into defense responses against Verticillium dahliae infection revealed by a quantitative proteomic analysis in Arabidopsis thaliana
Min Wu A B C # , Qiulin Li D # , Guixian Xia B , Yongshan Zhang D and Fuxin Wang
A B E *
+ Author Affiliations
- Author Affiliations
A College of Life Sciences, Hebei University, Baoding 071002, China.
B Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
C University of Chinese Academy of Sciences, Beijing 100049, China.
D State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences, Anyang, Henan 455000, China.
E Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, China.
* Correspondence to: wangfuxin@hbu.edu.cn
# These authors contributed equally to this paper
Handling Editor: Calum Wilson
Functional Plant Biology 49(11) 980-994 https://doi.org/10.1071/FP22006
Submitted: 9 January 2022 Accepted: 8 July 2022 Published: 1 August 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution 4.0 International License (CC BY)
Abstract
Verticillium wilt is a highly destructive fungal disease that attacks a broad range of plants, including many major crops. However, the mechanism underlying plant immunity toward Verticillium dahliae is very complex and requires further study. By combining bioinformatics analysis and experimental validation, we investigated plant defence responses against V. dahliae infection in the model plant Arabidopsis thaliana L. A total of 301 increased and 214 decreased differentially abundant proteins (DAPs) between mock and infected wild type (WT) plants were acquired and bioinformatics analyses were then conducted and compared (increased vs decreased) in detail. In addition to the currently known mechanisms, several new clues about plant immunity against V. dahliae infection were found in this study: (1) exosome formation was dramatically induced by V. dahliae attack; (2) tryptophan-derived camalexin and cyanogenic biosynthesis were durably promoted in response to infection; and (3) various newly identified components were activated for hub immunity responses. These new clues provide valuable information that extends the current knowledge about the molecular basis of plant immunity against V. dahliae infection.
Keywords: Arabidopsis, camalexin, cyanogenic biosynthesis, exosome, iTRAQ, plant immunity, proteome, Verticillium dahliae.
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