Identifying the genetic control of salinity tolerance in the bread wheat landrace Mocho de Espiga Branca
Chana Borjigin A B , Rhiannon K. Schilling A B C , Nathaniel Jewell B D , Chris Brien
B D , Juan Carlos Sanchez-Ferrero A B , Paul J. Eckermann A B , Nathan S. Watson-Haigh A B E , Bettina Berger B D , Allison S. Pearson A B F and Stuart J. Roy A B G *
+ Author Affiliations
- Author Affiliations
A Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia.
B School of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
C Department of Primary Industries and Regions, South Australian Research and Development Institute, Urrbrae, SA 5064, Australia.
D Australian Plant Phenomics Facility, The Plant Accelerator, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
E South Australian Genomics Centre, South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
F ARC Centre of Excellence in Plant Energy Biology, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
G ARC Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia.
Functional Plant Biology 48(11) 1148-1160 https://doi.org/10.1071/FP21140
Submitted: 3 May 2021 Accepted: 4 August 2021 Published: 4 October 2021
© 2021 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC)
Abstract
Salinity tolerance in bread wheat is frequently reported to be associated with low leaf sodium (Na+) concentrations. However, the Portuguese landrace, Mocho de Espiga Branca, accumulates significantly higher leaf Na+ but has comparable salinity tolerance to commercial bread wheat cultivars. To determine the genetic loci associated with the salinity tolerance of this landrace, an F2 mapping population was developed by crossing Mocho de Espiga Branca with the Australian cultivar Gladius. The population was phenotyped for 19 salinity tolerance subtraits using both non-destructive and destructive techniques. Genotyping was performed using genotyping-by-sequencing (GBS). Genomic regions associated with salinity tolerance were detected on chromosomes 1A, 1D, 4B and 5A for the subtraits of relative and absolute growth rate (RGR, AGR respectively), and on chromosome 2A, 2B, 4D and 5D for Na+, potassium (K+) and chloride (Cl−) accumulation. Candidate genes that encode proteins associated with salinity tolerance were identified within the loci including Na+/H+ antiporters, K+ channels, H+-ATPase, calcineurin B-like proteins (CBLs), CBL-interacting protein kinases (CIPKs), calcium dependent protein kinases (CDPKs) and calcium-transporting ATPase. This study provides a new insight into the genetic control of salinity tolerance in a Na+ accumulating bread wheat to assist with the future development of salt tolerant cultivars.
Keywords: chloride, genotyping-by-sequencing, landrace, Na, phenotyping, plant growth, quantitative trait loci, QTL, salt tolerance, sodium, wheat.
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