Pollen viability as a potential trait for screening heat-tolerant wheat (Triticum aestivum L.)
Mamrutha Harohalli Masthigowda
A * , Davinder Sharma A B , Rinki Khobra A , Gopalareddy Krishnappa A , Hanif Khan A , Sanjay Kumar Singh A , Gyanendra Singh A and Gyanendra Pratap Singh A
+ Author Affiliations
- Author Affiliations
A ICAR-Indian Institute of Wheat and Barley Research, Karnal, 132001 Haryana, India.
B The Institute for cereal crops improvement, Tel Aviv University, Tel Aviv 6997801, Israel.
Functional Plant Biology 49(7) 625-633 https://doi.org/10.1071/FP21096
Submitted: 31 March 2021 Accepted: 16 February 2022 Published: 11 March 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
High temperature during reproductive stage of winter crops causes sterility of pollen grains and reduced yield. It is essential to find the genotypes with higher pollen viability, as it is most sensitive to temperature extremes. A field study was conducted with wheat (Triticum aestivum L.) genotypes to understand the effect of high temperature on pollen viability and grain yield for 2 years under timely (TS) and late sown (LS) conditions. A strong correlation was observed between higher pollen viability and higher grain yield under heat stress condition. Genotypes like K7903, HD2932, WH730 and RAJ3765 showed higher pollen viability, whereas DBW17, HUW468, RAJ4014 and UP2425 had lower pollen viability under LS condition. Further, the quantification of antioxidant enzymes activity mainly, Super oxide dismutase (SOD), Catalase (CAT), Peroxidase (POD) and Glutathione peroxidase (GPX) has showed significant variation among study genotypes. Thus, the identified high pollen viability genotypes can serve as a potential source for trait based breeding under heat stress in wheat. The present study is a first of its kind to assess more number of wheat genotypes for pollen viability and antioxidants activity under field condition. It also confirms that pollen viability can be used as a potential trait to screen genotypes for heat stress tolerance in wheat.
Keywords: aniline blue, antioxidant enzymes, CAT activity, GPX activity, heat stress, pollen viability, SOD activity, wheat.
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