Seed nanopriming by silicon oxide improves drought stress alleviation potential in wheat plants
Prabha Rai-Kalal A , Rupal S. Tomar A and Anjana Jajoo
A B C
+ Author Affiliations
- Author Affiliations
A School of Life Sciences, Devi Ahilya University, Indore, India.
B School of Biotechnology, Devi Ahilya University, Indore, India.
C Corresponding author. Email: anjanajajoo@hotmail.com
Functional Plant Biology 48(9) 905-915 https://doi.org/10.1071/FP21079
Submitted: 13 March 2021 Accepted: 6 April 2021 Published: 17 May 2021
Abstract
The present study explored the effectiveness of SiO2 nanoparticles (NPs) as seed priming agent (15 mg L–1) to improve drought tolerance in the wheat cultivar HI 1544. Seed germination studies showed significant enhancement in the rate of seed germination, seedling growth and vigour, seed water uptake, and amylase activity in nanoprimed (NP) seeds compared with unprimed (UP) seeds. Pot experiments using wheat plants subjected to drought stress showed that SiO2 nanopriming enhanced the ability of wheat plants to withstand water deficit conditions by balancing the production of reactive oxygen species and the activity of enzymatic antioxidants like peroxidase, catalase, and superoxide dismutase. Investigations of photosynthetic parameters showed that under drought conditions, nanoprimed plants had a higher number of active reaction centres, high absorbance, trapping, and electron transport rates compared with unprimed plants. These results suggest the effects of silicon nanopriming in enhancing drought tolerance in wheat by alleviating drought induced inhibition of plant photosynthetic machinery and maintaining biochemical balance, ultimately resulting in an increase in biomass production. Results revealed the use of silicon oxide nanopriming to be a good option to increase drought tolerance in wheat plants.
Keywords: antioxidants, drought tolerance, drought stress, nanopriming, photosynthesis, wheat, silicon dioxide.
References
Agathokleous E, Feng Z, Iavicoli I, Calabrese EJ (2019) The two faces of nanomaterials: a quantification of hormesis in algae and plants. Environment International 131, 105044| The two faces of nanomaterials: a quantification of hormesis in algae and plants.Crossref | GoogleScholarGoogle Scholar | 31362152PubMed |
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276–287.
| Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.Crossref | GoogleScholarGoogle Scholar | 4943714PubMed |
Bustos DV, Hasan AK, Reynolds MP, Calderini DF (2013) Combining high grain number and weight through a DH-population to improve grain yield potential of wheat in high-yielding environments. Field Crops Research 145, 106–115.
| Combining high grain number and weight through a DH-population to improve grain yield potential of wheat in high-yielding environments.Crossref | GoogleScholarGoogle Scholar |
Ceppi MG, Oukarroum A, Cicek N, Strasser RJ, Schansker G (2012) The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves: a study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress. Physiologia Plantarum 144, 277–288.
| The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves: a study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress.Crossref | GoogleScholarGoogle Scholar | 22121914PubMed |
Dimkpa CO, Bindraban PS (2016) Fortification of micronutrients for efficient agronomic production: a review. Agronomy for Sustainable Development 36, 7
| Fortification of micronutrients for efficient agronomic production: a review.Crossref | GoogleScholarGoogle Scholar |
Ghorbanpour M, Mohammadi H, Kariman K (2020) Nanosilicon-based recovery of barley (Hordeum vulgare) plants subjected to drought stress. Environmental Science. Nano 7, 443–461.
| Nanosilicon-based recovery of barley (Hordeum vulgare) plants subjected to drought stress.Crossref | GoogleScholarGoogle Scholar |
Greger M, Landberg T, Vaculík M (2018) Silicon influences soil availability and accumulation of mineral nutrients in various plant species. Plants 7, 41
| Silicon influences soil availability and accumulation of mineral nutrients in various plant species.Crossref | GoogleScholarGoogle Scholar |
Hattori T, Inanaga S, Arakib H, Ping A, Moritac S, Miroslava L, Alexander L (2005) Application of silicon enhanced drought tolerance in Sorghum bicolor. Physiologia Plantarum 123, 459–466.
| Application of silicon enhanced drought tolerance in Sorghum bicolor.Crossref | GoogleScholarGoogle Scholar |
Karimi J, Mohsenzadeh S (2016) Effects of silicon oxide nanoparticles on growth and physiology of wheat seedlings. Russian Journal of Plant Physiology: a Comprehensive Russian Journal on Modern Phytophysiology 63, 119–123.
| Effects of silicon oxide nanoparticles on growth and physiology of wheat seedlings.Crossref | GoogleScholarGoogle Scholar |
Kataria S, Jain M, Tripathi DK, Singh VP (2020) Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean. Physiologia Plantarum 168, 422–436.
Kummerová M, Slovák L, Mázanková J, Holoubek I (1997) The effect of fluoranthene on the germination of plants. Environmental Toxicology and Chemistry 60, 235–244.
| The effect of fluoranthene on the germination of plants.Crossref | GoogleScholarGoogle Scholar |
Li X, Jiang H, Liu F, Cai J, Dai T, Cao W, Jiang D (2013) Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin. Plant Growth Regulation 71, 31–40.
| Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin.Crossref | GoogleScholarGoogle Scholar |
Li Y, Zhou C, Wang S, et al (2019) Phytotoxicity and oxidative effects of typical quaternary ammonium compounds on wheat (Triticum aestivum L.) seedlings. Environmental Science and Pollution Research International 26, 25985–25999.
| Phytotoxicity and oxidative effects of typical quaternary ammonium compounds on wheat (Triticum aestivum L.) seedlings.Crossref | GoogleScholarGoogle Scholar | 31273661PubMed |
Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt stressed barley (Hordeum vulgare L.). Journal of Plant Physiology 160, 1157–1164.
| Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt stressed barley (Hordeum vulgare L.).Crossref | GoogleScholarGoogle Scholar | 14610884PubMed |
Liang Y, Nikolic M, Bélanger R, Gong H, Song A (2015) ‘Silicon in agriculture: from theory to practice.’ (Springer, Science+Business Media: Dordrecht, Netherlands)
Liu F, Shahnazari A, Andersen MN, Jacobsen SE, Jensen CR (2006) Physiological responses of potato (Solanum tuberosum L.) to partial root-zone drying: ABA signaling, leaf gas exchange, and water use efficiency. Journal of Experimental Botany 57, 3727–3735.
| Physiological responses of potato (Solanum tuberosum L.) to partial root-zone drying: ABA signaling, leaf gas exchange, and water use efficiency.Crossref | GoogleScholarGoogle Scholar | 16982651PubMed |
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry 193, 265–275.
| Protein measurement with the folin phenol reagent.Crossref | GoogleScholarGoogle Scholar | 14907713PubMed |
Mahakham W, Sarmah AK, Maensiri S, et al (2017) Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles. Scientific Reports 7, 8263
| Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles.Crossref | GoogleScholarGoogle Scholar | 28811584PubMed |
Mathur S, Sharma MP, Jajoo A (2018) Improved photosynthetic efficacy of maize (Zea mays) plants with arbuscular mycorrhizal fungi (AMF) under high temperature stress. Journal of Photochemistry and Photobiology. B, Biology 180, 149–154.
| Improved photosynthetic efficacy of maize (Zea mays) plants with arbuscular mycorrhizal fungi (AMF) under high temperature stress.Crossref | GoogleScholarGoogle Scholar | 29425887PubMed |
Mathur S, Tomar RS, Jajoo A (2019) Arbuscular mycorrhizal fungi (AMF) protects photosynthetic apparatus of wheat under drought stress. Photosynthesis Research 139, 227–238.
| Arbuscular mycorrhizal fungi (AMF) protects photosynthetic apparatus of wheat under drought stress.Crossref | GoogleScholarGoogle Scholar | 29982909PubMed |
Parent C, Capelli N, Dat J (2008) Reactive oxygen species, stress and cell death in plants. Comptes Rendus Biologies 331, 255–261.
| Reactive oxygen species, stress and cell death in plants.Crossref | GoogleScholarGoogle Scholar | 18355747PubMed |
Parveen A, Liu W, Hussain S, Asghar J, Perveen S, Xiong Y (2019) Silicon priming regulates morpho-physiological growth and oxidative metabolism in maize under drought stress. Plants 8, 431
| Silicon priming regulates morpho-physiological growth and oxidative metabolism in maize under drought stress.Crossref | GoogleScholarGoogle Scholar |
Praba ML, Cairns JE, Babu RC, Lafitte HR (2009) Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat. Journal Agronomy Crop Science 195, 30–46.
| Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat.Crossref | GoogleScholarGoogle Scholar |
Qados AMSA, Moftah AE (2015) Influence of silicon and nano-silicon on germination, growth and yield of faba bean (Vicia faba L.) under salt stress conditions. American Journal of Experimental Agriculture 5, 509–524.
| Influence of silicon and nano-silicon on germination, growth and yield of faba bean (Vicia faba L.) under salt stress conditions.Crossref | GoogleScholarGoogle Scholar |
Rai-Kalal P, Jajoo A (2021) Priming with zinc oxide nanoparticles improve germination and photosynthetic performance in wheat. Plant Physiology and Biochemistry 160, 341–351.
| Priming with zinc oxide nanoparticles improve germination and photosynthetic performance in wheat.Crossref | GoogleScholarGoogle Scholar | 33548801PubMed |
Rangwala T, Angurbala B, Vyas N, Gupta R (2019) Beneficial role of soluble silica in enhancing chlorophyll content in onion leaves. Current Agriculture Research Journal 7, 358–367.
| Beneficial role of soluble silica in enhancing chlorophyll content in onion leaves.Crossref | GoogleScholarGoogle Scholar |
Rao MV, Palijyath G, Ormrod DP (1996) Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology 110, 125–136.
| Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 8587977PubMed |
Rastogi A, Tripathi DK, Yadav S, et al. (2019) Application of silicon nanoparticles in agriculture. 3 Biotech 9, 90
| Application of silicon nanoparticles in agriculture.Crossref | GoogleScholarGoogle Scholar | 30800601PubMed |
Ratnakumar P, Khan I, Minhas PS, et al. (2016) Can plant bio-regulators minimize crop productivity losses caused by drought, salinity and heat stress? An integrated review. Journal of Applied Botany and Food Quality 89, 113–125.
Sapre S, Vakharia D (2019) Effect of silicon under water deficit on antioxidant enzymes activities at different phenological stages in wheat (Triticum aestivum L.). Indian Journal of Agricultural Biochemistry 32, 30–36.
| Effect of silicon under water deficit on antioxidant enzymes activities at different phenological stages in wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar |
Sharma A, Kumar V, Shahzad B, et al (2020) Photosynthetic response of plants under different abiotic stresses: a review. Journal of Plant Growth Regulation 39, 509–531.
| Photosynthetic response of plants under different abiotic stresses: a review.Crossref | GoogleScholarGoogle Scholar |
Suriyaprabha R, Karunakaran G, Yuvakkumar R, Rajendran V, Kannan N (2012) Silica nanoparticles for increased silica availability in maize (Zea mays. L) seeds under hydroponic conditions. Current Nanoscience 8, 902–908.
| Silica nanoparticles for increased silica availability in maize (Zea mays. L) seeds under hydroponic conditions.Crossref | GoogleScholarGoogle Scholar |
Takahashi S, Badger MR (2011) Photoprotection in plants: a new light on photosystem II damage. Trends in Plant Science 16, 53–60.
| Photoprotection in plants: a new light on photosystem II damage.Crossref | GoogleScholarGoogle Scholar | 21050798PubMed |
Tomar RS, Jajoo A (2014) Fluoranthene, a polycyclic aromatic hydrocarbon, inhibits light as well as dark reactions of photosynthesis in wheat (Triticum aestivum). Ecotoxicology and Environmental Safety 109, 110–115.
| Fluoranthene, a polycyclic aromatic hydrocarbon, inhibits light as well as dark reactions of photosynthesis in wheat (Triticum aestivum).Crossref | GoogleScholarGoogle Scholar | 25173746PubMed |
Tomar RS, Jajoo A (2015) Photomodified fluoranthene exerts more harmful effects compared to intact fluoranthene by inhibiting growth and photosynthetic processes in wheat. Ecotoxicology and Environmental Safety 122, 31–36.
| Photomodified fluoranthene exerts more harmful effects compared to intact fluoranthene by inhibiting growth and photosynthetic processes in wheat.Crossref | GoogleScholarGoogle Scholar | 26186727PubMed |
Tripathi DK, Singh S, Singh VP, Prasad SM, Dubey NK, Chauhan DK (2017) Silicon nanoparticles more effectively alleviated UV-B stress than silicon in wheat (Triticum aestivum) seedlings. Plant Physiology and Biochemistry 110, 70–81.
| Silicon nanoparticles more effectively alleviated UV-B stress than silicon in wheat (Triticum aestivum) seedlings.Crossref | GoogleScholarGoogle Scholar | 27470120PubMed |
Uarrota VG, Stefen DLV, Leolato LS, Gindri DM, Nerling D (2019) Revisiting carotenoids and their role in plant stress responses: From biosynthesis to plant signaling mechanisms during stress. In ‘Antioxidants and antioxidant enzymes in higher plants’. (Eds. D Gupta, J Palma, F Corpas) pp. 207–232. (Springer: Cham, Switzerland)
Verma S, Mishra SN (2005) Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology 162, 669–677.
| Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system.Crossref | GoogleScholarGoogle Scholar | 16008089PubMed |
Wojtyla Ł, Lechowska K, Kubala S, Garnczarska M (2016) Different modes of hydrogen peroxide action during seed germination. Frontiers in Plant Science 7, 66
| Different modes of hydrogen peroxide action during seed germination.Crossref | GoogleScholarGoogle Scholar | 26870076PubMed |
Yang Y, Liu Q, Wang GX, Wang XD, Guo JY (2010) Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress. New Forests 39, 231–243.
| Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress.Crossref | GoogleScholarGoogle Scholar |
Yuan XK, Yang ZQ, Li YX, Liu Q, Han W (2016) Effects of different levels of water stress on leaf photosynthetic characteristics and antioxidant enzyme activities of greenhouse tomato. Photosynthetica 54, 28–39.
| Effects of different levels of water stress on leaf photosynthetic characteristics and antioxidant enzyme activities of greenhouse tomato.Crossref | GoogleScholarGoogle Scholar |
Zhang FQ, Wang YS, Lou ZP, Dong JD (2007) Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plants seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere 67, 44–50.
| Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plants seedlings (Kandelia candel and Bruguiera gymnorrhiza).Crossref | GoogleScholarGoogle Scholar | 17123580PubMed |
Zhang X, Wang X, Zhong J, et al (2016) Drought priming induces thermo-tolerance to post-anthesis high-temperature in offspring of winter wheat. Environmental and Experimental Botany 127, 26–36.
| Drought priming induces thermo-tolerance to post-anthesis high-temperature in offspring of winter wheat.Crossref | GoogleScholarGoogle Scholar |
Zivcak M, Kalaji HM, Shao HB, Olsovska K, Brestic M (2014) Photosynthetic proton and electron transport in wheat leaves under prolonged moderate drought stress. Journal of Photochemistry and Photobiology. B, Biology 137, 107–115.
| Photosynthetic proton and electron transport in wheat leaves under prolonged moderate drought stress.Crossref | GoogleScholarGoogle Scholar | 24508481PubMed |
