MYB59 transcription factor behaves differently in metallicolous and non-metallicolous populations of Arabidopsis halleri
Elisa Fasani A , Giovanni DalCorso A and Antonella Furini
A B
+ Author Affiliations
- Author Affiliations
A Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
B Corresponding author. Email: antonella.furini@univr.it
Functional Plant Biology 48(9) 916-923 https://doi.org/10.1071/FP20356
Submitted: 13 November 2020 Accepted: 9 April 2021 Published: 11 May 2021
Abstract
In Arabidopsis thaliana (L.) Heynh., MYB59 transcription factor participates in regulating Ca homeostasis and signal transduction and is induced by Cd excess. To investigate its role in the facultative metallophyte Arabidopsis halleri ssp. halleri (L.) O’Kane and Al-Shehbaz, MYB59 expression was investigated under Cd treatment or Ca depletion in three populations belonging to distinct phylogeographic units (metallicolous PL22 and I16 and non-metallicolous I29), and compared with the expression in A. thaliana. In control conditions, MYB59 transcription in A. thaliana and the non-metallicolous population I29 follow a comparable trend with higher expression in roots than shoots, whereas in metallicolous populations I16 and PL22 its expression is similar in roots and shoots, suggesting a convergent evolution associated with adaptation to metalliferous environments. After 6 h of Ca depletion, MYB59 transcript levels were very high in I16 and PL22 populations, indicating that the adaptation to metalliferous environments requires tightly regulated Ca homeostasis and signalling. Cd treatment caused variability in MYB59 expression. In I29, MYB59 expression, as in A. thaliana, is likely associated to stress response, whereas its modulation in the two metallicolous populations reflects the different strategies for Cd tolerance and accumulation. In conclusion, MYB59 regulation in A. halleri is part of the network linking mineral nutrition and Cd tolerance/accumulation.
Keywords: MYB59 transcription factor, metal hyperaccumulation, Arabidopsis halleri, cadmium, calcium homeostasis, calcium signaling.
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