Epigenetic memory and growth responses of the clonal plant Glechoma longituba to parental recurrent UV-B stress
Xiaoyin Zhang A , Cunxia Li A , Dan Tie A , Jiaxin Quan A , Ming Yue A and Xiao Liu A BA Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China.
B Corresponding author. Email: liuxiao@nwu.edu.cn
Functional Plant Biology 48(8) 827-838 https://doi.org/10.1071/FP20303
Submitted: 30 September 2020 Accepted: 10 March 2021 Published: 6 April 2021
Journal Compilation © CSIRO 2021 Open Access CC BY-NC-ND
Abstract
The responses of plants to recurrent stress may differ from their responses to a single stress event. In this study, we investigated whether clonal plants can remember past environments. Parental ramets of Glechoma longituba (Nakai) Kuprian were exposed to UV-B stress treatments either once or repeatedly (20 and 40 repetitions). Differences in DNA methylation levels and growth parameters among parents, offspring ramets and genets were analysed. Our results showed that UV-B stress reduced the DNA methylation level of parental ramets, and the reduction was enhanced by increasing the number of UV-B treatments. The epigenetic variation exhibited by recurrently stressed parents was maintained for a long time, but that of singly stressed parents was only short-term. Moreover, clonal plants responded to different UV-B stress treatments with different growth strategies. The one-time stress was a eustress that increased genet biomass by increasing offspring leaf allocation and defensive allocation in comparison to the older offspring. In contrast, recurring stress was a distress that reduced genet biomass, increased the biomass of storage stolons, and allocated more defensive substances to the younger ramets. This study demonstrated that the growth of offspring and genets was clearly affected by parental experience, and parental epigenetic memory and the transgenerational effect may play important roles in this effect.
Keywords: environmental stress, phenotypic plasticity, DNA methylation, stress memory, transgenerational effect.
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