Arabidopsis cysteine-rich trans-membrane module (CYSTM) small proteins play a protective role mainly against heat and UV stresses
Janak Raj Joshi A B , Vikram Singh A and Haya Friedman
A C
+ Author Affiliations
- Author Affiliations
A Department of Postharvest Science of Fresh Produce, Agricultural Research Organisation (ARO), The Volcani Centre, Bet Dagan, Israel.
B Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, Israel.
C Corresponding author. Email: hayafr@agri.gov.il.
Functional Plant Biology 47(3) 195-202 https://doi.org/10.1071/FP19236
Submitted: 22 August 2019 Accepted: 15 October 2019 Published: 3 February 2020
Abstract
The genomes of Arabidopsis and other plants contain cysteine-rich small protein of unknown function, harbouring a transmembrane module (CYSTM proteins). In this work we show that the transcript of one gene (At1g05340) encoding a CYSTM protein is induced mainly by heat and to a lesser extent by UV, but less by NaCl or sorbitol. A functional analysis of At1g05340 and its paralog At2g32210 using T-DNA insertional mutants revealed a decrease in seedlings root length, and a lower PSII efficiency in mature plant, due to heat stress and to a lesser extent due to UV stress, in comparison to the effect on wild-type plants. The sensitivity of these mutants to salt or osmotic stresses did not differ from wild type response, indicating a specific function for these genes in heat and UV. Heat and UV increased reactive oxygen species levels in wild type; however, the levels were higher in the mutant line than in wild type due to heat treatment, but was similar in the mutant lines and wild type due to UV stress. Taken together, our results suggest that these small cysteine-rich proteins are necessary for thermotolerance and protection from UV exposure. The proteins encoded by these genes most likely, act in heat stress by reducing reactive oxygen species level by yet unknown mechanism.
Additional keywords: At1g05340, At2g32210, cysteine-rich transmembrane module protein, PSII efficiency, ROS, thermotolerance.
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