Contributions of cryptochromes and phototropins to stomatal opening through the day
Fang Wang
A , T. Matthew Robson A , Jorge J. Casal B C , Alexey Shapiguzov A D and Pedro J. Aphalo A E
+ Author Affiliations
- Author Affiliations
A Viikki Plant Science Centre (ViPS), Organismal and Evolutionary Biology (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Finland.
B IFEVA, Facultad de Agronomía, Universidad de Buenos Aires and CONICET, Av. San Martín 4453,1417 Buenos Aires, Argentina.
C Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–CONICET,1405 Buenos Aires, Argentina.
D Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street, 35, 127276 Moscow, Russia.
E Corresponding author. Email: pedro.aphalo@helsinki.fi
Functional Plant Biology 47(3) 226-238 https://doi.org/10.1071/FP19053
Submitted: 16 February 2019 Accepted: 19 October 2019 Published: 12 February 2020
Abstract
The UV-A/blue photoreceptors phototropins and cryptochromes are both known to contribute to stomatal opening (Δgs) in blue light. However, their relative contributions to the maintenance of gs in blue light through the whole photoperiod remain unknown. To elucidate this question, Arabidopsis phot1 phot2 and cry1 cry2 mutants (MTs) and their respective wild types (WTs) were irradiated with 200 μmol m–2 s–1 of blue-, green- or red-light (BL, GL or RL) throughout a 11-h photoperiod. Stomatal conductance (gs) was higher under BL than under RL or GL. Under RL, gs was not affected by either of the photoreceptor mutations, but under GL gs was slightly lower in cry1 cry2 than its WT. Under BL, the presence of phototropins was essential for rapid stomatal opening at the beginning of the photoperiod, and maximal stomatal opening beyond 3 h of irradiation required both phototropins and cryptochromes. Time courses of whole-plant net carbon assimilation rate (Anet) and the effective quantum yield of PSII photochemistry (ΦPSII) were consistent with an Anet-independent contribution of BL on gs both in phot1 phot2 and cry1 cry2 mutants. The changing roles of phototropins and cryptochromes through the day may allow more flexible coordination between gs and Anet.
Additional keywords: Arabidopsis thaliana, blue light, diurnal pattern, gas exchange, green light, photosynthesis, red light, stomata.
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