Genome-wide identification of the glutathione transferase superfamily in the model organism Brachypodium distachyon
Ágnes Gallé
A C , Dániel Benyó B , Jolán Csiszár A and János Györgyey B
+ Author Affiliations
- Author Affiliations
A Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
B Institute of Plant Biology, Biological Research Centre, H-6726 Szeged, Hungary.
C Corresponding author. Email: gallea@bio.u-szeged.hu
Functional Plant Biology 46(11) 1049-1062 https://doi.org/10.1071/FP19023
Submitted: 22 February 2019 Accepted: 2 July 2019 Published: 2 October 2019
Abstract
The detoxification of harmful metabolites can determine the effectiveness of plant stress responses. Scavenging some of these toxic stress by-products through the reduced form of glutathione is catalysed by members of the glutathione transferase (GST) enzyme superfamily. The involvement of these enzymes was studied in the model organism Brachypodium distachyon (L.) P.Beauv. Bd21 and in its derivative Bd21-3, a more drought tolerant line. Osmotic stress treatment resulted in a decrease in the water potential of both Brachypodium genotypes, the difference between the control and treated plant’s ψw decreased by the last sampling day in Bd21-3, suggesting some degree of adaptation to the applied osmotic stress. Increased GST activity revealed a severe defence reaction against the harmful imbalance of the redox environment. Screening for the gene sequences led to the identification of 91 full-length or partial GST sequences. Although purple false brome has a relatively small genome, the number of identified GST genes was almost as high as the number predicted in wheat. The estimation of GST expression showed stress-induced differences: higher expression levels or the fast induction of BdGSTF8, BdGSTU35 and BdGSTU42 gene products presumably indicate a strong detoxification under osmotic stress.
Additional keywords: drought stress, drought tolerance, gene expression, glutathione S-transferase, osmotic stress.
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