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Plant function and evolutionary biology
RESEARCH ARTICLE

Large-scale stage-specific regulation of gene expression during host–pathogen interactions in CSP44 bread wheat carrying APR gene Lr48

Neelu Jain A , Sushma Rani A , Chanchal Sharma B C , Nivedita Sinha A , Anupam Singh A , Jai Bhagwan Sharma A , Pramod Prasad D , Gautam Saripalli B , Pradeep Kumar Sharma B , Harindra Singh Balyan B , Pushpendra Kumar Gupta https://orcid.org/0000-0001-7638-6171 B F and Kumble Vinod Prabhu A E
+ Author Affiliations
- Author Affiliations

A Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.

B Chaudhary Charan Singh University, Meerut 250004, UP, India.

C Department of Biotechnology, College of Engineering, Daegu University, Gyeongsan City, Gyeongbook 38453, South Korea.

D Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Shimla 171002, India.

E Protection of Plant Varieties and Farmers’ Rights Authority, Govt. of India, Ministry of Agriculture & Farmers Welfare, New Delhi 110012 (India).

F Corresponding author. Email: pkgupta@ccsuniversity.ac.in

Functional Plant Biology 47(3) 203-225 https://doi.org/10.1071/FP18336
Submitted: 24 December 2018  Accepted: 18 October 2019   Published: 3 February 2020

Abstract

Genome-wide transcriptome analysis was undertaken in a leaf-rust resistant bread wheat line CSP44 (selected from Australian cv. Condor) carrying the adult plant resistance (APR) gene Lr48. Two pre-adult plant (P-AP) susceptible stages (S48 and S96) and two adult plant (AP) resistant stages (R48 and R96) were used for RNA-seq. At the susceptible P-AP stage (during S48 to S96), expression increased in 2062 genes, and declined in 130 genes; 1775 of 2062 differentially expressed genes (DEGs) also exhibited high expression during early incompatible stage R48. Comparison of S96 with R96 showed that the expression of 80 genes was enhanced and that of 208 genes declined at the AP stage. At the resistant AP stage (during R48 to R96), expression of mere 25 genes increased and that of 126 genes declined. Apparently, the resistance during late adult stage (R96) is caused by regulation of the expression of relatively fewer genes, although at pre-adult stage (S48 to S96), expression of large number of genes increased; expression of majority of these genes kept on increasing during adult stage at R48 also. These and other results of the present study suggest that APR may mimic some kind of systemic acquired resistance (SAR). The host-specific DEGs belonged to 10 different classes including genes involved in defence, transport, epigenetics, photosynthesis, genes encoding some transcription factors etc. The pathogen (Puccinia triticina) specific DEGs (including three genes encoding known biotrophic effectors) seem to help the pathogen in infection/growth through large-scale stage-specific enhanced expression of host’s genes. A putative candidate gene for Lr48 containing protein kinase domain (its ortholog in rice encoding OsWAK8) was also identified.

Additional keywords: adult plant resistance, expression profiling, leaf rust.


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