Amplification of abiotic stress tolerance potential in rice seedlings with a low dose of UV-B seed priming
Dhanya T. Thomas A and Jos T. Puthur A B
+ Author Affiliations
- Author Affiliations
A Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, C.U. Campus P.O., Kerala-673635, India.
B Corresponding author. Email: jtputhur@yahoo.com
Functional Plant Biology 46(5) 455-466 https://doi.org/10.1071/FP18258
Submitted: 5 October 2018 Accepted: 21 January 2019 Published: 27 February 2019
Abstract
UV-B radiation is a major abiotic stress factor that adversely affects the growth and productivity of crop plants including rice (Oryza sativa L.). However, on the other hand, lower doses of UV-B radiation applied to seeds can have a priming effect on plants emerging from it. In this study, seeds of O. sativa var. kanchana were primed with UV-B radiation (6 kJ m–2) and were further subjected to NaCl, polyethylene glycol 6000 (PEG) and UV-B stress. The effects of UV-B priming in imparting NaCl, PEG and UV-B stress tolerance to rice seedlings were analysed through various photosynthetic features and antioxidative mechanisms. PSI and PSII activity levels as well as chl a fluorescence were found to be significantly higher in the UV-B primed and unstressed seedlings. When stress (NaCl, PEG and high UV-B) was imposed, increased PSI and PSII activity levels, chl a fluorescence and metabolite accumulation (proline, total phenolics and sugar) as well as nonenzymatic (ascorbate and glutathione) and enzymatic (superoxide dismutase, catalase, ascorbate peroxidase) antioxidants were recorded in UV-B primed and NaCl-stressed plants followed by UV-B primed and UV-B–stressed plants, and primed and PEG-stressed, compared with unprimed and stressed conditions. The results indicate that UV-B priming in rice seedlings effectively enhances the NaCl stress tolerance potential in rice to a greater extent than UV-B and PEG stress tolerance potential. The cost-effectiveness of UV-B seed priming is predominantly clear from the differing tolerance responses of rice seedlings exposed to different stress conditions.
Additional keywords: antioxidants, Oryza sativa, photosynthesis, salt stress.
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