Blue and red light effects on stomatal oscillations
Trevor Ballard A , David Peak B and Keith Mott
A C
+ Author Affiliations
- Author Affiliations
A Department of Biology, Utah State University, 5305 Old Main Hill, Logan UT, 84322, USA.
B Department of Physics, Utah State University, 4415 Old Main Hill, Logan UT, 84322, USA.
C Corresponding author. Email: keith.mott@usu.edu
Functional Plant Biology 46(2) 146-151 https://doi.org/10.1071/FP18104
Submitted: 20 April 2018 Accepted: 12 September 2018 Published: 24 October 2018
Abstract
The response of stomata to red and blue light was investigated using small fibre optics (66 µm diameter) to control light levels on a single pair of guard cells without affecting the surrounding tissue. Low intensity red light (50 µmol m–2 s–1) applied to the entire leaf caused stomata to oscillate continuously for several hours with no apparent decrease in amplitude with time. Adding low intensity blue light (50 µmol m–2 s–1) caused stomata to stop oscillating, but oscillations resumed when the blue light was removed. Adding the same intensity of red light to an oscillating leaf changed the amplitude of the oscillations but did not stop them. When blue light was added to a single guard cell pair (using a fibre optic) in a red-light-illuminated leaf, the stoma formed by that pair stopped oscillating, but adjacent stomata did not. Red light added to a single guard cell pair did not stop oscillations. Finally, blue light applied through a fibre optic to areas of leaf without stomata caused proximal stomata to stop oscillating, but distal stomata continued to oscillate. The data suggest that blue light affects stomata via direct effects on guard cells as well as by indirect effects on other cells in the leaf.
Additional keywords: blue light, guard cells, red light, stomata, oscillations.
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