DNA synthesis pattern, proteome, and ABA and GA signalling in developing seeds of Norway maple (Acer platanoides)
Aleksandra M. Staszak A C , Monika Rewers B , Elwira Sliwinska B , Ewelina A. Klupczyńska A and Tomasz A. Pawłowski
A D
+ Author Affiliations
- Author Affiliations
A Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
B Laboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Kaliskiego Avenue. 7, 85-789 Bydgoszcz, Poland.
C Present address: Department of Plant Physiology, Institute of Biology, Faculty of Biology and Chemistry, University of Białystok, Ciołkowskiego 1J, 15-245 Białystok, Poland.
D Corresponding author. Email: tapawlow@man.poznan.pl
Functional Plant Biology 46(2) 152-164 https://doi.org/10.1071/FP18074
Submitted: 28 March 2018 Accepted: 13 September 2018 Published: 30 October 2018
Abstract
Mature seeds of Norway maple exhibit desiccation tolerance and deep physiological dormancy. Flow cytometry, proteomics, and immunodetection have been combined to investigate seed development of this species. DNA content analysis revealed that cell cycle/endoreduplication activity differs between seed organs and developmental stages. In the embryo axis, the proportion of the nuclei with the highest DNA content (4C) increases at the beginning of maturation (17 weeks after flowering; WAF), and then is stable until the end of maturation, to increase again after drying. In cotyledons, during maturation endopolyploid nuclei (8C) occur and the intensity of endoreduplication increases up to 21 WAF, and then is stable until development is completed. In dry mature seeds, the proportion of 4C nuclei is high, and reaches 36% in the embryo axis and 52% in cotyledons. Proteomic studies revealed that energy and carbon metabolism, fatty acid biosynthesis, storage and antioxidant proteins are associated with seed development. Study of the ABI5 protein, a transcription factor involved in ABA signalling, and the RGL2 protein, a repressor of the GA signalling indicates that the highest accumulation of these proteins occurs in fully-matured and dried seeds. It is suggested that this increase in accumulation can be associated with completion of maturation, mainly with desiccation and dormancy acquisition.
Additional keywords: cell cycle, endoreduplication, desiccation, flow cytometry, seed dormancy, tree.
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