Enhancing antioxidant systems by exogenous spermine and spermidine in wheat (Triticum aestivum) seedlings exposed to salt stress
Abdelaleim I. ElSayed A F , Mohammed S. Rafudeen B , Mohamed A. M. El-hamahmy C , Dennis C. Odero D and M. Sazzad Hossain E
+ Author Affiliations
- Author Affiliations
A Biochemistry Department, Faculty of Agriculture, Zagazig University, 44519 Zagazig, Egypt.
B Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa.
C Department of Agricultural Botany, Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt.
D Everglades Research and Education Centre, University of Florida-IFAS, 3200 East Palm Beach Road, Belle Glade, FL 33430, USA.
E Department of Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University, Universitätsstr.25, D-33615, Bielefeld, Germany.
F Corresponding author. Email: aelsayed@zu.edu.eg
Functional Plant Biology 45(7) 745-759 https://doi.org/10.1071/FP17127
Submitted: 30 April 2017 Accepted: 22 January 2018 Published: 13 February 2018
Abstract
Plants have evolved complex mechanisms to mitigate osmotic and ionic stress caused by high salinity. The effect of exogenous spermine (Spm) and spermidine (Spd) on defence responses of wheat seedlings under NaCl stress was investigated by measuring antioxidant enzyme activities and the transcript expression of corresponding genes. Exogenous Spm and Spd decreased the level of malondialdehyde, increased chlorophyll and proline contents, and modulated PSII activity in wheat seedlings under salt stress. Spermidine alleviated negative effects on CO2 assimilation induced by salt stress in addition to significantly increasing the activity and content of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). It appears Spd conferred salinity tolerance in wheat seedlings by enhancing photosynthetic capacity through regulation of gene expression and the activity of key CO2 assimilation enzymes. Exogenous Spm regulated activities of different antioxidant enzymes (catalase, glutathione reductase, dehydroascorbate reductase, ascorbate peroxidase, and superoxide dismutase) and efficiently modulate their transcription levels in wheat seedlings under salt stress. It is likely that Spm plays a key role in alleviating oxidative damage of salt stress by adjusting antioxidant enzyme activities in plants. In addition, exogenous Spd increased transcript level of spermine synthase under salt stress. Salinity stress also caused an increase in transcript levels of diamine oxidase (DAO) and polyamine oxidase (PAO). Exogenous Spd application resulted in a marked increase in free Spd and Spm contents under saline conditions. These results show that exogenous Spd and Spm effectively upregulated transcriptional levels of antioxidant enzyme genes and improved the defence response of plants under salt stress.
Additional keywords: antioxidant enzymes, gene expression, salt stress, spermine, spermidine, wheat.
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