An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression
Donna Glassop A C , Jiri Stiller A , Graham D. Bonnett A , Christopher P. L. Grof B and Anne L. Rae A
+ Author Affiliations
- Author Affiliations
A CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, Qld 4067, Australia.
B School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
C Corresponding author. Email: donna.glassop@csiro.au
Functional Plant Biology 44(8) 795-808 https://doi.org/10.1071/FP17073
Submitted: 11 November 2016 Accepted: 11 May 2017 Published: 1 June 2017
Abstract
The role of ShSUT1 in sucrose mobilisation and storage in sugarcane was investigated by employing RNAi technology to reduce the expression of this gene. Transcript profiling in non-transformed plants showed an alignment between expression and sucrose concentration, with strongest expression in source leaves and increasing expression through the daylight period of a diurnal cycle. Five transgenic plant lines were produced with reduced ShSUT1 expression ranging from 52 to 92% lower than control plants. Differential suppression of ShSUT1 sequence variants in the highly polyploid sugarcane genome were also investigated. Amplicon sequencing of the ShSUT1 variants within the transgenic lines and controls showed no preferential suppression with only minor differences in the proportional expression of the variants. A range of altered sugar, fibre and moisture contents were measured in mature leaf and internode samples, but no phenotype was consistently exhibited by all five transgenic lines. Phenotypes observed indicate that ShSUT1 does not play a direct role in phloem loading. ShSUT1 is likely involved with retrieving sucrose from intercellular spaces for transport and storage.
Additional keywords: allele, polyploidy, Saccharum, sucrose accumulation.
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