Stomata control is changed in a chlorophyll b-free barley mutant
Elena V. Tyutereva A , Valeria A. Dmitrieva A , Alexey L. Shavarda B C and Olga V. Voitsekhovskaja A D
+ Author Affiliations
- Author Affiliations
A Laboratory of Molecular and Ecological Physiology, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 Saint-Petersburg, Russia.
B Laboratory of Analytical Phytochemistry, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 Saint-Petersburg, Russia.
C Research Resource Centre ‘Molecular and Cell Technologies’, Saint-Petersburg State University, Universitetskaya nab. 7-9, 199034 Saint-Petersburg, Russia.
D Corresponding author. Email: ovoitse@binran.ru
Functional Plant Biology 45(4) 453-463 https://doi.org/10.1071/FP17056
Submitted: 23 February 2017 Accepted: 7 October 2017 Published: 13 November 2017
Abstract
The barley (Hordeum vulgare L.) chlorina f2 3613 mutant exhibits low photosynthesis and slow growth. This results from downregulation of the levels of photosynthetic antenna proteins caused by the absence of chl b, the major regulator of photosynthetic antennae in land plants. Here, we demonstrate that, when grown in the field in full sunlight, this mutant displays a changed pattern of stomatal responses compared with the parental wild-type cultivar Donaria. However, stomatal regulation of chlorina f2 3613 plants was restored when plants were placed under a shade cover for several days. The shade cover reduced incident PAR from 2000–2200 μmol m–2 s–1 to 800–880 μmol m–2 s–1 as measured at noon. Contents of ABA, the xanthophyll precursors of ABA biosynthesis and minor antenna proteins, as well as reactive oxygen species levels in stomata and the sensitivity of stomata to exogenously supplied ABA, were determined in leaves of wild-type Donaria and chlorina f2 3613 before and after shading. The results support the view that the restoration of stomatal control in barley chlorina f2 3613 is correlated with an increase in the levels of the minor antenna protein Lhcb6, which has recently been implicated in the enhancement of stomatal sensitivity to ABA in Arabidopsis thaliana (L.) Heynh.
Additional keywords: chlorina, Hordeum vulgare, photosynthetic antennae, shading.
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