Salt stress responses in a geographically diverse collection of Eutrema/Thellungiella spp. accessions
Yang Ping Lee A B , Christian Funk A , Alexander Erban A , Joachim Kopka A , Karin I. Köhl A , Ellen Zuther A and Dirk K. Hincha A C
+ Author Affiliations
- Author Affiliations
A Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14 476 Potsdam, Germany.
B Present address: FELDA Global Ventures Research and Development Sdn Bhd, Level 14, Menara Felda, Platinum Park, No 11, Persiaran KLCC, 50 088 Kuala Lumpur, Malaysia.
C Corresponding author. Email: hincha@mpimp-golm.mpg.de
This paper originates from presentations made at the 1st ISF-BIDR workshop ‘Surviving the extremes: Abiotic stress tolerance in extremophile plants’ held at Ben-Gurion University of the Negev, 2–5 February 2015.
Functional Plant Biology 43(7) 590-606 https://doi.org/10.1071/FP15285
Submitted: 12 September 2015 Accepted: 10 December 2015 Published: 16 February 2016
Abstract
Salinity strongly impairs plant growth and development. Natural genetic variation can be used to dissect complex traits such as plant salt tolerance. We used 16 accessions of the halophytic species Eutrema salsugineum (previously called Thellungiella salsuginea (Pallas) O.E.Schulz, Thellungiella halophila (C.A.Meyer) O.E. Schulz and Thellungiella botschantzevii D.A.German to investigate their natural variation in salinity tolerance. Although all accessions showed survival and growth up to 700 mM NaCl in hydroponic culture, their relative salt tolerance varied considerably. All accessions accumulated the compatible solutes proline, sucrose, glucose and fructose and the polyamines putrescine and spermine. Relative salt tolerance was not correlated with the content of any of the investigated solutes. We compared the metabolomes and transcriptomes of Arabidopsis thaliana (L. Heynh.) Col-0 and E. salsugineum Yukon under control and salt stress conditions. Higher content of several metabolites in Yukon compared with Col-0 under control conditions indicated metabolic pre-adaptation to salinity in the halophyte. Most metabolic salt responses in Yukon took place at 200 mM NaCl, whereas few additional changes were observed between 200 and 500 mM. The opposite trend was observed for the transcriptome, with only little overlap between salt-regulated genes in the two species. In addition, only about half of the salt-regulated Yukon unigenes had orthologues in Col-0.
Additional keywords: extremophiles, gas chromatography–mass spectrometry, microarrays.
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