Salt tolerance of the halophyte Limonium delicatulum is more associated with antioxidant enzyme activities than phenolic compounds
Aymen Souid A , Morena Gabriele B , Vincenzo Longo B , Laura Pucci B , Lorenza Bellani B C , Abderrazak Smaoui A , Chedly Abdelly A and Karim Ben Hamed A D
+ Author Affiliations
- Author Affiliations
A Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, BP 901, Hammam Lif 2050, Tunisia.
B National Research Council, Institute of Biology and Agricultural Biotechnology (IBBA), Pisa Unit, Research Area of Pisa, Via Moruzzi 1, 56124 Pisa, Italy.
C Department of Life Sciences, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
D Corresponding author. Email: kbenhamed@yahoo.fr
Functional Plant Biology 43(7) 607-619 https://doi.org/10.1071/FP15284
Submitted: 12 September 2015 Accepted: 6 March 2016 Published: 13 May 2016
Abstract
In this work we studied the effect of salinity (ranging from 50 to 500 mM NaCl) on the physiological and the antioxidant responses of the local halophyte Limonium delicatulum Kuntze. We based our analysis on 12 biochemical assays that are commonly used to measure the antioxidant responses under stress such as oxidative stress markers, enzymes activities and polyphenolic compounds. Our aim was to study parameters that are strongly correlated with the growth response to salinity. Results showed two different growth responses depending on the concentration of NaCl in the medium. Under 50 to 200 mM, the growth was stimulated before it decreased significantly at 300–500 mM. L. delicatulum revealed a good aptitude to maintain photosynthetic machinery by increasing the concentrations of photosynthetic pigments, which is essential for the stabilisation of photosystems and the photosynthesis process under optimal NaCl concentration. Their breakdown at higher salinity decreased the photosynthetic performance of plants resulting in growth inhibition. Moreover, to reduce the damaging effect of oxidative stress and to tolerate the accumulation of salt ions, L. delicatulum induced the activities of their antioxidant enzymes more than their contents in polyphenolic compounds.
Additional keywords: antioxidant enzymes, halophyte, Limonium delicatulum, polyphenols, salinity.
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