Biochemical and molecular characterisation of salt-induced poor grain filling in a rice cultivar
Binay B. Panda A , Alok K. Badoghar A , Sudhanshu Sekhar A , Ekamber Kariali B , Pravat K. Mohapatra B and Birendra P. Shaw A C
+ Author Affiliations
- Author Affiliations
A Environmental Biotechnology Laboratory, Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, Odisha, India.
B School of Life Sciences, Sambalpur University, Sambalpur, 768019, Odisha, India.
C Corresponding author. Email: bpshaw@ils.res.in
Functional Plant Biology 43(3) 266-277 https://doi.org/10.1071/FP15229
Submitted: 4 August 2015 Accepted: 6 November 2015 Published: 15 December 2015
Abstract
Despite the prevalence of poor grain filling in rice (Oryza sativa L.) under abiotic stress, the reason for this is largely unexplored. Application of 0.75% NaCl to a salt-sensitive rice cultivar at late booting resulted in a >20% yield loss. Spikelets per panicle and the percentage of filled grain decreased significantly in response to NaCl application. The inhibitory effect of NaCl on grain filling was greater in basal than in apical spikelets. Sucrose synthase (SUS) activity was positively correlated with grain weight. The transcript levels of the SUS isoforms differed greatly: the levels of SUS2 increased significantly in response to salt; those of SUS4 decreased drastically. Gene expression studies of starch synthase and ADP-glucose pyrophosphorylase showed that the decreased transcript levels of one isoform was compensated by an increase in those of the other. Salt application also significantly increased the gene expression of the ethylene receptors and the ethylene signalling proteins. The increase in their transcript levels was comparatively greater in basal than in apical spikelets. Significant enhancement in the transcript levels of the ethylene receptors and the increase in the production of ethylene indicated that the salt-induced inhibition of grain filling might be mediated by ethylene. Additionally, the inhibition of chromosomal endoreduplication mediated by decreased transcript levels of B-type cyclin could explain poor grain filling under salt stress. A significant increase in the transcript levels of the ethylene-responsive factors in the spikelets during grain filling in response to salt indicated their possible protective role in grain filling under stress.
Additional keywords: abiotic stress, chromosomal endoreduplication, ethylene receptor,NaCl salinity, Oryza sativa, sucrose synthase.
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