The source of nitrogen (NH4+ or NO3–) affects the concentration of oxalate in the shoots and the growth of Atriplex nummularia (oldman saltbush)
Hussein Al Daini A B , Hayley C. Norman C D , Paul Young C and Edward G. Barrett-Lennard B D E F
+ Author Affiliations
- Author Affiliations
A School of Animal Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C CSIRO Animal, Food and Health Sciences and Sustainable Agriculture Flagship, Private Bag 5, Wembley, WA 6913, Australia.
D Centre for Ecohydrology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
E Department of Agriculture and Food of WA, South Perth, WA 6151, Australia.
F Corresponding author. Email: egbarrettlennard@agric.wa.gov.au
Functional Plant Biology 40(10) 1057-1064 https://doi.org/10.1071/FP13060
Submitted: 19 March 2013 Accepted: 27 April 2013 Published: 3 June 2013
Abstract
Atriplex nummularia Lindl. (oldman saltbush) is a halophytic shrub used widely as a forage for ruminant production in saline farming systems. However, it can contain high concentrations of oxalate in the leaves, which may cause calcium deficiency in grazing animals. We hypothesised that supplying NH4+ instead of NO3– to a clone of this species would decrease oxalate concentrations in the shoots, and also decrease plant growth. Oxalate concentrations were measured in plants in the field, and a glasshouse experiment was conducted in which plants were grown with 10 mM NO3– or NH4+, with 50, 200 or 500 mM NaCl. The field survey showed effects of site (P < 0.001), with average oxalate concentrations in shoots varying between 2.4 and 6.4% dry mass (DM). In the glasshouse, oxalate concentrations and plant growth were both affected by N-source and salinity (P < 0.001). Averaged across salinities, plants grown with NH4+ for 24 days had only 43% of the shoot DM but 25% of the oxalate concentration of plants grown with NO3–. We discuss the effects of N-source on oxalate concentrations, the implications of this for halophyte growth, and the opportunity to select halophytes with lower oxalate and higher nutritive value for livestock.
Additional keywords: growth analysis, halophytes, nitrogen nutrition, nitrogen uptake, osmotic adjustment, salt tolerance.
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