Cloning, functional characterisation and transgenic manipulation of vitamin E biosynthesis genes of wheat
Neetu Chaudhary A and Paramjit Khurana A B
+ Author Affiliations
- Author Affiliations
A Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India.
B Corresponding author. Email: param@genomeindia.org
Functional Plant Biology 40(11) 1129-1136 https://doi.org/10.1071/FP12265
Submitted: 5 September 2012 Accepted: 20 May 2013 Published: 5 July 2013
Abstract
Tocochromanols are an important group of plastidic lipophilic antioxidants that form an essential part of human diet and play important functions in photosynthetic organisms by protecting them from photo-oxidation, lipid peroxidation and membrane damage. Molecular genetics and genomics-based approaches have revealed the genes required for synthesis of these compounds in model organisms like rice, Arabidopsis and Synechocystis. To create a positive impact on human nutrition and health, the levels of total and specific tocochromanols have been altered in various agricultural crops by metabolic engineering. To understand the mechanisms involved in higher tocochromanol levels of wheat seeds and its germ, the tocochromanol biosynthesis pathway was investigated in wheat. The focus of this research was towards isolation of genes involved in wheat tocochromanol biosynthesis, and homologous and heterologous transgenic manipulation to alter their content and composition. Functional characterisation of TaHydroxyphenylpyruvate dioxygenase and Taγ-Tocopherol methyltransferase-overexpressing transgenic Arabidopsis plants revealed alterations in tocochromanol content and composition, which suggests better growth of these plants in the presence of sorbitol. TaHydroxyphenylpyruvate dioxygenase-overexpressing transgenic wheat, Triticum aestivum L. plants also showed 2.4-fold increase in tocochromanol content, which may have nutritional as well as antioxidative roles. Further characterisation and field trials of these transgenic lines can provide us more insight about the antioxidative roles of tocochromanols.
Additional keywords: osmotic stress, tocochromanol, Triticum aestivum.
References
Chaudhary N, Khurana P (2009) Vitamin E biosynthesis genes in rice: molecular characterization, expression profiling and comparative phylogenetic analysis. Plant Science 177, 479–491.| Vitamin E biosynthesis genes in rice: molecular characterization, expression profiling and comparative phylogenetic analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVKktr%2FM&md5=3a06626050fcb0d8a2bd0c100dd1f80fCAS |
Cho EA, Lee CA, Kim YS, Baek SH, de los Reyes BG, Yun SJ (2004) Expression of γ-tocopherol methyltransferase transgene improves tocopherol composition in lettuce (Latuca sativa L.). Molecules and Cells 19, 16–22.
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735–743.
| Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1M7mvVagsQ%3D%3D&md5=2d7dce8087879a64bd0f5c4ee5957cd1CAS | 10069079PubMed |
Dat J, Vandenabeele S, Vranova E, van Montagu M, Inze D, van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences 57, 779–795.
| Dual action of the active oxygen species during plant stress responses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXksFyisrk%3D&md5=6d5f355e09b48e75470bf67a0e38b8c7CAS | 10892343PubMed |
DellaPenna D (2005) A decade of progress in understanding vitamin E synthesis in plants. Journal of Plant Physiology 162, 729–737.
| A decade of progress in understanding vitamin E synthesis in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntVSmtbw%3D&md5=7008a85ce017f7eb66c686769ed7dba6CAS | 16008096PubMed |
DellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annual Review of Plant Biology 57, 711–738.
| Vitamin synthesis in plants: tocopherols and carotenoids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosVKht7Y%3D&md5=2296387bd06b7e0293a65cfa1329caf7CAS | 16669779PubMed |
Dufourmantel N, Dubald M, Matringe M, Canard H, Garcon F, Job C, Kay E, Wisniewski JP, Ferullo JM, Pelissier B, Sailland A, Tissot G (2007) Generation and characterization of soybean and marker free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance. Plant Biotechnology Journal 5, 118–133.
| Generation and characterization of soybean and marker free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitV2jurw%3D&md5=c4c374f4a523e8fba2381baf8c3996feCAS | 17207262PubMed |
Falk J, Andersen G, Kernebeck B, Krupinska K (2003) Constitutive overexpression of barley 4-hydroxyphenylpyruvate dioxygenase in tobacco results in elevation of the vitamin E content in seeds but not in leaves. FEBS Letters 540, 35–40.
| Constitutive overexpression of barley 4-hydroxyphenylpyruvate dioxygenase in tobacco results in elevation of the vitamin E content in seeds but not in leaves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXislCitLc%3D&md5=887f29c31bc50d2fb98de0d298e8357fCAS | 12681479PubMed |
Falk J, Brosch M, Schafer A, Braun S, Krupinska K (2005) Characterization of transplastomic tobacco plants with a plastid localized barley 4-hydroxyphenylpyruvate dioxygenase. Journal of Plant Physiology 162, 738–742.
| Characterization of transplastomic tobacco plants with a plastid localized barley 4-hydroxyphenylpyruvate dioxygenase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntVSmtb0%3D&md5=1ce6c115f1170972a90733b9a963e9b9CAS | 16008097PubMed |
Fryer MJ (1992) The antioxidant effects of thylakoid vitamin E (α-tocopherol). Plant, Cell & Environment 15, 381–392.
| The antioxidant effects of thylakoid vitamin E (α-tocopherol).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38Xks1Knu7c%3D&md5=320bf53824ad65de22c929442eee3c8eCAS |
Genty B, Briantais J, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta 990, 87–92.
| The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXhsFWntL4%3D&md5=c7e023b607e8d6002006223a88c3674dCAS |
Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51, 463–499.
| Plant cellular and molecular responses to high salinity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlsVymt7s%3D&md5=c67a7b95a8761aa0d533df99ebe4cd38CAS | 15012199PubMed |
Hooque MA, Arima S (2000) Evaluation of salt damage through cell membrane stability monitored by electrolyte leakage in water chestnut (Trapa sp.) Bulletin of Faculty of Agriculture 85, 141–146.
Kagan RM, Clarke S (1994) Widespread occurrence of three sequence motifs in diverse S-adenosylmethionine-dependent methyltransferases suggests a common structure for these enzymes. Archives of Biochemistry and Biophysics 310, 417–427.
| Widespread occurrence of three sequence motifs in diverse S-adenosylmethionine-dependent methyltransferases suggests a common structure for these enzymes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXktVert7w%3D&md5=8818231edb19d8d1d903f57287825112CAS | 8179327PubMed |
Kim YJ, Seo HY, Park TI, Baek SH, Shin WC, Kim HS, Kim JG, Choi YE, Yun SJ (2005) Enhanced biosynthesis of α-tocopherol in transgenic soybean by introducing γ-TMT gene. Journal of Plant Biotechnology 7, 1–7.
Moran GR (2005) 4-Hydroxyphenylpyruvate dioxygenase. Archives of Biochemistry and Biophysics 433, 117–128.
| 4-Hydroxyphenylpyruvate dioxygenase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVCgt7fN&md5=6560d4d460e9f61cf007f88b24629a5cCAS | 15581571PubMed |
Murashige T, Skoog F (1962) A revised media for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| A revised media for rapid growth and bioassays with tobacco tissue cultures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXksFKm&md5=00e945f2329f915ef516a05110061063CAS |
Nagarajan S, Bansal KC (1986) Measurement of cellular membrane thermostability to evaluate foliage heat tolerance of potato. Potato Research 29, 163–167.
| Measurement of cellular membrane thermostability to evaluate foliage heat tolerance of potato.Crossref | GoogleScholarGoogle Scholar |
Okada K, Shimura Y (1990) Reversible root tip rotation in Arabidopsis seedlings induced by obstacle-touching stimulus. Science 250, 274–276.
| Reversible root tip rotation in Arabidopsis seedlings induced by obstacle-touching stimulus.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cvjsFCktA%3D%3D&md5=5016b01149a4c52b40039947540448eeCAS | 17797309PubMed |
Panfili G, Fratianni A, Irano M (2003) Normal phase high performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereals. Journal of Agricultural and Food Chemistry 51, 3940–3944.
| Normal phase high performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktVKqsrg%3D&md5=4c615f6bcc39c7901db3d1c0cb5872cfCAS | 12822927PubMed |
Patnaik D, Vishnudasan D, Khurana P (2006) Agrobacterium-mediated transformation of mature embryos of Triticum aestivum and T. durum. Current Science 91, 307–317.
Rippert P, Scimemi C, Dubald M, Matringe M (2004) Engineering plant shikimate pathway for production of tocotrienol and improving herbicide resistance. Plant Physiology 134, 92–100.
| Engineering plant shikimate pathway for production of tocotrienol and improving herbicide resistance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVagtbY%3D&md5=1c6b65c47ff4ded09495ae559b3024a1CAS | 14684842PubMed |
Sambrook JT, Fritsch EF, Maniatis T (1989) ‘Molecular cloning: a laboratory manual.’ 2nd edn. (Cold Spring Harbor Laboratory: New York)
Shintani D, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282, 2098–2100.
| Elevating the vitamin E content of plants through metabolic engineering.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXotVyisrc%3D&md5=20f53c7d21afc07b2c451b15d8682f0eCAS | 9851934PubMed |
Sullivan CY, Ross MY (1979) Selections for drought and heat resistance in grain sorghum. In ‘Stress physiology in crop plants’. pp. 263–281. (Wiley: New York)
Tavva VS, Kim YH, Kagan IA, Dinkins RD, Kim KH, Collins GB (2007) Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ-tocopherol methyltransferase gene. Plant Cell Reports 26, 61–70.
| Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ-tocopherol methyltransferase gene.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht12gs7jI&md5=e9d2c7ff23ba13efc07ac4c241a2c8dfCAS | 16909228PubMed |
Tsegaye Y, Shintani DK, DellaPenna D (2002) Overexpression of the enzyme p-hydroxyphenylpyruvate dioxygenase in Arabidopsis and its relation to tocopherol biosynthesis. Plant Physiology and Biochemistry 40, 913–920.
| Overexpression of the enzyme p-hydroxyphenylpyruvate dioxygenase in Arabidopsis and its relation to tocopherol biosynthesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XovFCisb0%3D&md5=b81e47cfb92bdd1ffb04029693b2e878CAS |
Upadhyaya E, Davies TD, Larsen MH, Walser RH, Sankhla N (1990) Uniconazole-induced thermotolerance in soybean seedling root tissue. Physiologia Plantarum 79, 78–84.
| Uniconazole-induced thermotolerance in soybean seedling root tissue.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXkslaqtLs%3D&md5=87fe0a99b30836037c1d6b0c5bc361beCAS |
Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL (2003) Engineering vitamin E content: from Arabidopsis mutant to soy oil. The Plant Cell 15, 3007–3019.
| Engineering vitamin E content: from Arabidopsis mutant to soy oil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVSgsw%3D%3D&md5=c1cf5e45923ba09ed4f68e9c044de0c3CAS | 14630966PubMed |
Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35, 753–759.
| Proline accumulation in plants: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1aisrfP&md5=0ee3f762d1e8f9f2650e618694cca205CAS | 18379856PubMed |
