Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea)
Tong Wang A B C , Xiao-Ping Chen A , Hai-Fen Li A , Hai-Yan Liu A , Yan-Bin Hong A , Qing-Li Yang C , Xiao-Yuan Chi C , Zhen Yang C , Shan-Lin Yu C , Ling Li B and Xuan-Qiang Liang A B D
+ Author Affiliations
- Author Affiliations
A Crops Research Institute, Guangdong Academy of Agricultural Sciences, Wushan 510640, Guangzhou, China.
B College of Life Science, South China Normal University, Shipai 510631, Guangzhou, China.
C Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Licang 266100, Qingdao, China.
D Corresponding author. Email: liang-804@163.com
Functional Plant Biology 40(3) 292-303 https://doi.org/10.1071/FP12143
Submitted: 17 May 2012 Accepted: 4 October 2012 Published: 20 November 2012
Abstract
Pre-harvest aflatoxin contamination caused by Aspergillus favus is a major concern in peanut. However, little is known about the resistance mechanism, so the incorporation of resistance into cultivars with commercially-acceptable genetic background has been slowed. To identify resistance-associated genes potentially underlying the resistance mechanism, we compared transcriptome profiles in resistant and susceptible peanut genotypes under three different treatments: well watered, drought stress and both A. flavus and drought stress using a customised NimbleGen microarray representing 36 158 unigenes. Results showed that the profile of differentially expressed genes (DEGs) displayed a similar pattern of distribution among the functional classes between resistant and susceptible peanuts in response to drought stress. Under A. flavus infection with drought stress, a total of 490 unigenes involved in 26 pathways were differentially expressed in the resistant genotype YJ1 uniquely responding to A. flavus infection, in which 96 DEGs were related to eight pathways: oxidation reduction, proteolysis metabolism, coenzyme A biosynthesis, defence response, signalling, oligopeptide transport, transmembrane transport and carbohydrate biosynthesis/metabolism. Pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that eight networks were significantly associated with resistance to A. flavus infection in resistant genotype YJ1 compared with susceptible Yueyou7. To validate microarray analysis, 15 genes were randomly selected for real-time RT–PCR analysis. The results provided in this study may enhance our understanding of the pre-harvest peanut–A. flavus interaction and facilitate to develop aflatoxin resistant peanut lines in future breeding programs.
Additional keywords: aflatoxin, microarray, peanut, resistance-associated genes.
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