Arbuscular mycorrhisation with Glomus irregulare induces expression of potato PR homologues genes in response to infection by Fusarium sambucinum
Youssef Ismail A B and Mohamed Hijri A C
+ Author Affiliations
- Author Affiliations
A Université de Montréal, Département de sciences biologiques, Institut de recherche en biologie végétale (IRBV), 4101 rue Sherbrooke Est, Montréal, QC, H1X 2B2, Canada.
B Plant Pathology Unit, Department of Plant Protection, Desert Research Center, Cairo, Egypt.
C Corresponding author. Email: mohamed.hijri@umontreal.ca
Functional Plant Biology 39(3) 236-245 https://doi.org/10.1071/FP11218
Submitted: 28 September 2011 Accepted: 13 January 2012 Published: 21 February 2012
Abstract
Arbuscular mycorrhizal fungi (AMF) are symbiotic, root-inhabiting fungi colonising a wide range of vascular plant species. We previously showed that AMF modulate the expression of mycotoxin genes in Fusarium sambucinum. Here, we tested the hypothesis that AMF may induce defence responses in potato to protect against infection with F. sambucinum. We analysed the response of AMF-colonised potato plants to the pathogenic fungus F. sambucinum by monitoring the expression of defence-related genes ChtA3, gluB, CEVI16, OSM-8e and PR-1. In response to F. sambucinum infection, we found that the AMF treatment upregulated the expression of all defence genes except OSM-8e in potato roots at 72 and 120 h post infection (hpi). However, we found variable transcriptional regulation with gluB and CEVI16 in shoots at both times 72 and 120 hpi in AMF-colonisation and infected plants. Overall, differential regulation of defence-related genes in leaf tissues indicate that AMF are a systemic bio-inducer and their effect could extend into non-infected parts. Thus, AMF significantly suppressed disease severity of F. sambucinum on potato plants compared with those infected and non-mycorrhizal plants. Furthermore, the AMF treatment decreased the negative effects of F. sambucinum on biomass and potato tuber production.
Additional keywords: arbuscular mycorrhizal fungi, Fusarium sambucinum, gene expression, Glomus irregulare, induced resistance, mycotoxins, potatoes, PR proteins, qRT-PCR.
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