Analysis of differentially expressed genes in leaf rust infected bread wheat involving seedling resistance gene Lr28
Raman Dhariwal A , Shailendra Vyas B , Govindraj R. Bhaganagare C , Shailendra K. Jha C , Jitendra P. Khurana B , Akhilesh K. Tyagi D , Kumble V. Prabhu C , Harindra S. Balyan A and Pushpendra K. Gupta A E
+ Author Affiliations
- Author Affiliations
A Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250004, UP, India.
B Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
C National Phytotron Facility, Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
D National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
E Corresponding author. Email: pkgupta36@gmail.com
Functional Plant Biology 38(6) 479-492 https://doi.org/10.1071/FP10246
Submitted: 18 December 2010 Accepted: 24 March 2011 Published: 3 June 2011
Abstract
Genome-wide transcriptome analysis of seedling resistance to leaf rust conferred by Lr28 gene in wheat (Triticum aestivum L.) was conducted to identify differentially expressed genes during incompatible interaction. A virulent leaf rust race 77–5 was used for inoculation of resistant (HD2329 + Lr28) and susceptible (HD2329 – Lr28) wheat NILs and cDNA-AFLP analyses was carried out. As many as 223 differential transcripts appeared following leaf rust inoculation; these included 122 transcripts that appeared exclusively in resistant NIL, whereas 39 transcripts appeared both in resistant and susceptible NILs. Sequence analyses of 37 transcripts, which appeared in the resistant NIL revealed that 15 transcripts had homology with genes involved in protein synthesis, signal transduction, transport, disease resistance and metabolism. The functions of remaining 22 transcripts could not be determined; these included six novel genes reported for the first time in wheat. Specific primers could be designed for 18 of the 37 transcripts, which included genes with putative and unknown functions. Quantitative real time PCR analysis was conducted using these 18 pairs of primers. A majority (13) of these transcripts appeared within 48 h reaching a peak value at 96 h in resistant NIL signifying their role in providing leaf rust resistance.
Additional keywords: cDNA-AFLP analysis, compatible interaction, defense, incompatible interaction, Puccinia triticina, qRT-PCR analysis, TDFs.
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