Comparative genomics of two ecologically differential populations of Hibiscus tiliaceus under salt stress
Guili Yang A , Xiaoshu Chen A , Tian Tang A , Renchao Zhou A , Sufang Chen A , Weijing Li A , Jianhua Ouyang A , Lian He A and Shuhua Shi A B
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, The People’s Republic of China.
B Corresponding author. Email: lssssh@mail.sysu.edu.cn
Functional Plant Biology 38(3) 199-208 https://doi.org/10.1071/FP10228
Submitted: 24 November 2010 Accepted: 14 January 2011 Published: 29 March 2011
Abstract
Hibiscus tiliaceus L. is a mangrove associate that occupies the divergent environments of intertidal wetland (L population) and inland (T population). Thus, it is an ideal plant for the study of ecological adaptation and salt tolerance. In this study we compared responses of the two populations to salinity combining a global transcriptional analysis and physiological analysis. Microarray transcript profiling analysis showed both shared and divergent responses to salinity stress in the two populations. A total of 575 unigenes were identified as being salt-responsive in the two populations. Shared responses were exemplified by the regulated genes functioning in confining ribosomal functions, photosynthesis and cellular metabolism. A set of genes functioning in cellular transporting and cell detoxification and a crucial transcription factor AP2 domain-containing protein involved in environmental responsiveness, were differently expressed in the two populations. Physiological analysis showed that the L population was less susceptible to salt stress in photosynthesis and had a stronger capability of K+ : Na+ regulation than the T population. Both microarray and physiological data showed the L population possess higher fitness under high salinity, probably due to it its long-term adaptation to their native environment.
Additional keywords: adaptation, cDNA microarray, mangrove associate, salt stress.
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