Photosynthetic responses to chromosome doubling in relation to leaf anatomy in Lonicera japonica subjected to water stress
Wei-Dong Li A B , Dilip K. Biswas A , Hong Xu A , Chang-Qing Xu A , Xian-Zhong Wang C , Jia-Kun Liu D and Gao-Ming Jiang A E FA Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, P.R. China.
B School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, P.R. China.
C Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.
D Jiujianpeng Agricultural Technology Limited, Pingyi, Shandong 273300, P.R. China.
E State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, P.R. China.
F Corresponding author. Email: jianggm@126.com
Functional Plant Biology 36(9) 783-792 https://doi.org/10.1071/FP09022
Submitted: 23 January 2009 Accepted: 15 July 2009 Published: 3 September 2009
Abstract
Gas exchange, chlorophyll fluorescence, and contents of some metabolites in two Japanese honeysuckle (Lonicera japonica Thunb.) cultivars, Damaohua (2n = 2x) and Jiufengyihao (2n = 4x), were compared with explore the function of chromosome doubling under water stress conditions. Water stress significantly decreased net photosynthesis rate, stomatal conductance, and transpiration rate of both cultivars. It also decreased electron transport rate, effective quantum yield of Photosystem II, photochemical quenching, and starch content, but increased non-photochemical quenching and contents of total soluble sugars, proline, and malondialdehyde. However, the tetraploid cultivar showed higher resistance to water stress than the diploid, as indicated by the fact that gas exchange, chlorophyll fluorescence, and metabolites were less affected for the tetraploid than the diploid. Moreover, the tetraploid recovered more quickly than the diploid after re-watering. Morphological and anatomical analysis further revealed that the tetraploid possessed less whole plant leaf area, higher leaf mass per unit area, thicker epidermis (both upper and lower) and palisade tissue, as well as denser pubescence. All of those specialised structures caused by chromosome doubling might lead to greater capacity in coping with drought stress. Our findings suggest that the effect of chromosome doubling on drought resistance in L. japonica could attribute to the improvement of structure and photosynthesis-related traits.
Additional keywords: chlorophyll fluorescence, diploid, gas exchange, malondialdehyde, proline, tetraploid, total soluble sugars.
Acknowledgements
This study were financially supported by an Innovative Group Grant of National Science Foundation of China (30821062), the ‘973’ Project of China (2007CB106804) and National Key Technologies R&D Programs of China (2008BAD0B05). We thank Dr Eric S. J. Harris (Medical School, Harvard University, USA) for critical review of the manuscript and Drs Hong-En Jiang and Tie-Mei Yi (Institute of Botany, the Chinese Academy of Sciences, China) for their expert assistance on electron microscope scanning.
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