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RESEARCH ARTICLE
Contents Vol 35(12)

Identification of grapevine MLO gene candidates involved in susceptibility to powdery mildew

Angela Feechan A , Angelica M. Jermakow A , Laurent Torregrosa B , Ralph Panstruga C and Ian B. Dry A D
+ Author Affiliations
- Author Affiliations

A CSIRO Plant Industry, PO Box 350, Glen Osmond, SA 5064, Australia.

B UMR BEPC, campus-Agro-M/INRA, Montpellier, CEDEX 01, France.

C Max-Planck-Institut für Züchtungsforschung, Department of Plant Microbe Interactions, D-50829 Köln, Germany.

D Corresponding author. Email: ian.dry@csiro.au

Functional Plant Biology 35(12) 1255-1266 https://doi.org/10.1071/FP08173
Submitted: 20 June 2008  Accepted: 9 September 2008   Published: 16 December 2008

Abstract

The European cultivated grapevine, Vitis vinifera L., is a host for the powdery mildew pathogen Erisyphe necator, which is the most economically important fungal disease of viticulture. MLO proteins mediate powdery mildew susceptibility in the model plant species Arabidopsis and the crop plants barley and tomato. Seven VvMLO cDNA sequences were isolated from grapevine and were subsequently identified as part of a 17 member VvMLO gene family within the V. vinifera genome. Phylogenetic analysis of the 17 VvMLO genes in the grape genome indicated that the proteins they encode fall into six distinct clades. The expression of representative VvMLOs from each clade were analysed in a range of grape tissues, as well as in response to a range of biotic and abiotic factors. The VvMLOs investigated have unique, but overlapping tissue expression patterns. Expression analysis of VvMLO genes following E. necator infection identified four upregulated VvMLOs which are orthologous to the Arabidopsis AtMLO2, AtMLO6 and AtMLO12 and tomato SlMLO1 genes required for powdery mildew susceptibility. This suggests a degree of functional redundancy between the proteins encoded by these genes in terms of susceptibility to powdery mildew, and, as such, represent potential targets for modification to generate powdery mildew resistant grapevines.

Additional keywords: Erysiphe necator, grapevine, MLO, powdery mildew.


Acknowledgements

This work was funded by the Grape and Wine Research Development Corporation. We thank Nicole Kempster for excellent technical assistance. In addition we thank Dr Paul Boss and Dr Chris Davies for critical reading of the manuscript.


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