A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
Delphine Chinchilla A C , Florian Frugier A , Marcela Raices B D , Francisco Merchan A , Veronica Giammaria B , Pablo Gargantini B , Silvina Gonzalez-Rizzo A , Martin Crespi A E and Rita Ulloa B EA Institut des Sciences du Végétal (ISV), CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
B Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, INGEBI, FCEN-UBA, Vuelta de Obligado 2490, 2do piso, Buenos Aires, 1428 Argentina.
C Present address: Botanical Institute, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
D Present address: The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
E Corresponding authors. Emails: martin.crespi@isv.cnrs-gif.fr; rulloa@dna.uba.ar
Functional Plant Biology 35(1) 92-101 https://doi.org/10.1071/FP07209
Submitted: 31 August 2007 Accepted: 14 December 2007 Published: 25 January 2008
Abstract
A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development.
Additional keywords: Arabidopsis thaliana, tubulin phosphorylation.
Acknowledgements
We thank the Platform of Cell Biology and Spencer Brown for helping in confocal microscopy analysis. Screening of the AtAPK1 mutant was performed in the laboratory of David Bouchez (INRA Versailles, France) with the help of Fabienne Granier. Simone Poirier was involved in the generation of Medicago truncatula transgenics. Finally, we thank Rafael Pont-Lezica and Yves Henry for helpful suggestions and discussions. D.C. was recipient of a fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche. M.R., P.G. and V.G. were fellows of CONICET. This work was partially done in the frame of the Ecos-Sud Program (CNRS/France-SeCyT/Argentina).
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