Diversity and plasticity of C4 photosynthesis in Eleocharis (Cyperaceae)
Lesley R. Murphy A C , João Barroca A C , Vincent R. Franceschi A , Raymond Lee A , Eric H. Roalson A , Gerald E. Edwards A and Maurice S. B. Ku B DA School of Biological Sciences and Center for Integrated Biotechnology, Washington State University, Pullman, WA 99164-4236, USA.
B Institute of Agricultural Biotechnology, National Chiayi University, Chiayi 60004, Taiwan.
C These authors contributed equally to this paper.
D Corresponding author. Email: mku@mail.ncyu.edu.tw
Functional Plant Biology 34(7) 571-580 https://doi.org/10.1071/FP06296
Submitted: 11 November 2006 Accepted: 29 March 2007 Published: 4 July 2007
Abstract
Eleocharis contains many amphibious species, and displays diversity of photosynthetic mechanism (C3, C4 or C3-C4 intermediates). A unique feature of Eleocharis is the plasticity in the photosynthetic mechanism of some species in response to the environment. In this study, we have examined the culm anatomy and photosynthetic property of several Eleocharis species grown terrestrially and the changes in the newly produced culms over a short period time frame after switching from terrestrial to submerged condition. Eleocharis baldwinii (Torrey) Chapman is C4-like in terrestrial habitat, exhibiting O2 inhibition of photosynthesis with Rubisco expressed in both mesophyll and bundle sheath cells and PEPC strictly in the mesophyll cells, but switches to C3-C4 intermediacy when submerged. In addition to Eleocharis vivipara Link type 1 (which switches from C4-like to C3), two other photosynthetic types examined in this study were shown to have different responses to growth in either terrestrial or submerged conditions. E. vivipara type 2 is a typical C4 plant in the terrestrial habitat, but becomes a C3-C4 intermediate under submerged conditions. Further, terrestrially, E. vivipara type 3 is a C3-C4 intermediate, but when submerged the δ13C value increases to –6.7‰, indicating its use of bicarbonate as a major carbon source. The submerged form of this plant exhibited about three times higher photosynthetic O2 evolution rate, compared to the C3 species Eleocharis erythropoda Steudel. These Eleocharis species possess different molecular switches for regulating C4 gene expression in response to environmental stimuli both between different species, and in E. vivipara among different populations. The apparent expression of a bicarbonate transport system by E. vivipara type 3 while submerged represents a unique adaptation to low CO2 availability.
Acknowledgements
We thank Chuck Cody for cultivation of plants in the greenhouse and Dr Elena Voznesenskaya for assistance with figure preparation. We also thank the staff of the Electron Microscopy Center at Washington State University for use of facilities and assistance. This work has been partially supported by NSF grants 0515975, IBN-0236959 and NSF Isotope Facility Grant DBI-0116203.
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