The five families of sucrose-phosphate synthase genes in Saccharum spp. are differentially expressed in leaves and stem
C. P. L. Grof A B E , C. T. E. So A C , J. M. Perroux A B , G. D. Bonnett A B and R. I. Forrester DA CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Qld 4067, Australia.
B Cooperative Research Centre for Sugar Industry Innovation through Biotechnology, The University of Queensland, St Lucia, Qld 4072, Australia.
C School of Computer Science and Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
D CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia.
E Corresponding author. Email: Chris.Grof@csiro.au
Functional Plant Biology 33(6) 605-610 https://doi.org/10.1071/FP05283
Submitted: 24 November 2005 Accepted: 7 March 2006 Published: 1 June 2006
Abstract
Sucrose-phosphate synthase (SPS) is a key enzyme in the pathway of sucrose synthesis. Five different gene families encoding SPS have been reported in the Poaceae [Castleden CK, Aoki N, Gillespie VJ, MacRae EA, Quick WP, Buchner P, Foyer CH, Furbank RT, Lunn JE (2004) Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiology 135, 1753–1764]. Expression of the five families in leaf and stem tissues of Saccharum spp. at different stages of development was determined by quantitative real-time PCR. The type B and C families of SPS genes were predominantly expressed in both immature and mature leaves, whereas the two subfamilies making up the type D family were expressed at similar levels in all tissues examined. In the type A family, expression was lowest in leaves and increased from the meristem region down to internode 7 of the stem.
Keywords: gene expression, Saccharum, sucrose-phosphate synthase, SPS, sugarcane.
Acknowledgments
Many thanks to Donna Glassop, Mayling Goode, Janine Jarmey, Anne Rae, Hayati Iskandar and Mark Jackson for growing and harvesting sugarcane plant material. Thanks also to Anne Rae and Rosanne Casu for helpful comments on this manuscript. Thanks to Troy Stephenson for performing the sugar extractions. CTES was supported by the Australian Pastoral Trust CSIRO Summer Scholarship Program.
Aitken KS,
Jackson PA, McIntyre CL
(2005) A combination of AFLP and SSR markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. Theoretical and Applied Genetics 110, 789–801.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Aitken KS,
Jackson PA, McIntyre CL
(2006) Quantitative trait loci identified for sugar related traits in a sugarcane (Saccharum spp.) cultivar × Saccharum officinarum population. Theoretical and Applied Genetics 112, 1306–1317.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Campbell JA,
Hansen RW, Wilson JR
(1999) Cost-effective colorimetric microtitre plate enzymatic assays for sucrose, glucose and fructose in sugarcane tissue extracts. Journal of the Science of Food and Agriculture 79, 232–236.
| Crossref | GoogleScholarGoogle Scholar |
Castleden CK,
Aoki N,
Gillespie VJ,
MacRae EA,
Quick WP,
Buchner P,
Foyer CH,
Furbank RT, Lunn JE
(2004) Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiology 135, 1753–1764.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Chen S,
Hajirezaei M, Börnke F
(2005) Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves. Plant Physiology 139, 1163–1174.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gibon Y,
Blaesing OE,
Hannemann J,
Carillo P,
Hohne M,
Hendriks JHM,
Palacios N,
Cross J,
Selbig J, Stitt M
(2004) A robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays: comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness. The Plant Cell 16, 3304–3325.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Grivet L, Arruda P
(2002) Sugarcane genomics: depicting the complex genome of an important tropical crop. Current Opinion in Plant Biology 5, 122–127.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Grivet L,
D’Hont A,
Roques D,
Feldmann P,
Lanaud C, Glaszmann JC
(1996) RFLP mapping in cultivated sugarcane (Saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. Genetics 142, 987–1000.
| PubMed |
Ishimaru K,
Ono J, Kashiwagi T
(2004) Identification of a new gene controlling plant height in rice using the candidate-gene approach. Planta 218, 388–395.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Iskandar HM,
Simpson RS,
Casu RE,
Bonnett GD,
Maclean DJ, Manners JM
(2004) Comparison of reference genes for quantitative real-time PCR analysis of gene expression in sugarcane. Plant Molecular Biology Reporter 22, 325–337.
Langenkämper G,
Fung RWM,
Newcomb RD,
Atkinson RG,
Gardner RC, MacRae EA
(2002) Sucrose phosphate synthase genes in plants belong to three different families. Journal of Molecular Evolution 54, 322–332.
| PubMed |
Lunn JE, MacRae EA
(2003) New complexities in the synthesis of sucrose. Current Opinion in Plant Biology 6, 208–214.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Papageorgiou J,
Bartholomew HC, Doherty WOS
(1997) HPAE-pad: a rapid and precise method for sugar analysis. Proceedings of the Australian Society of Sugarcane Technologists 19, 379–386.
Rossi M,
Arujo P,
Paulet F,
Garsmeur O,
Dias V,
Hui C,
Van Sluys MA, D’Hont A
(2003) Genomic distribution and characterization of EST-derived resistance gene analogs (RGAs) in sugarcane. Molecular Genetics and Genomics 269, 406–419.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Sarquis JI,
Gonzalez H,
Sanchez de Jimenez E, Dunlap JR
(1998) Physiological traits associated with mass selection for improved yield in a maize population. Field Crops Research 56, 239–246.
| Crossref | GoogleScholarGoogle Scholar |
Stitt M
(1994) Manipulation of carbon partitioning. Current Opinion in Biotechnology 5, 137–143.
| Crossref | GoogleScholarGoogle Scholar |
Stitt M, Quick WP
(1989) Photosynthetic carbon partitioning: its regulation and possibilities for manipulation. Physiologia Plantarum 77, 633–641.
| Crossref |
Tanouye L,
Thom M,
Goldner W, Maretzki A
(1992) Partial purification and characterization of sucrose-P synthase from sugarcane storage tissue. Plant Physiology. Supplement 99, 56.
Vickers JE,
Grof CPL,
Bonnett GD,
Jackson PA,
Knight DP,
Roberts SE, Robinson SP
(2005) Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugar. Crop Science 45, 354–362.
Zhu YJ,
Komor E, Moore PH
(1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiology 115, 609–616.
| Crossref | GoogleScholarGoogle Scholar | PubMed |