Polypeptide metabolites secreted by the fungal pathogen Eutypa lata participate in Vitis vinifera cell structure damage observed in Eutypa dieback
Stéphane Octave A , Gabriel Roblin A , Magali Vachaud A and Pierrette Fleurat-Lessard A BA Université de Poitiers, Laboratoire de Physiologie et Biochimie Végétales, UMR CNRS 6161, Bâtiment Botanique, 40 Avenue du Recteur Pineau, F-86022 Poitiers, France.
B Corresponding author. Email: pfleurat@univ-poitiers.fr
Functional Plant Biology 33(3) 297-307 https://doi.org/10.1071/FP05230
Submitted: 20 September 2005 Accepted: 1 December 2005 Published: 2 March 2006
Abstract
Eutypa dieback is a devastating disease of Vitis vinifera L. caused by the fungal pathogen Eutypa lata. This wood-inhabiting fungus degrades tissues in the trunk and cordons of infected vines and induces symptoms in the foliage. These symptoms have been attributed to the production of toxic metabolites by the pathogen, in particular eutypine. Recently, we have isolated polypeptide compounds secreted by the fungus in artificial culture. The aims of this study were to examine the effects induced in leaves by applying polypeptides and eutypine to detached canes and to compare this to the changes in leaf structure induced by E. lata in the vineyard. In leaves taken from vines infected with E. lata, the changes in mesophyll cells indicate that the fungus has an effect on tissue remote from the infected area. The size of mesophyll cells decreased by more than half, starch content was reduced and tannins were abundant. Plastids, mitochondria and cell walls were highly modified. In leaves taken from healthy canes treated with polypeptides of E. lata, the structure of mesophyll cells was also modified. The cell size did not change, but the tannin content increased and modifications in plastids and mitochondria were similar to those observed in leaves taken from infected vines. The major effect was the complete disorganisation of cell walls. Eutypine had less effect on organelle structure and did not modify the cell wall. In canes treated with polypeptides, vessel-associated cells (VACs) were also damaged. Abundant tannins occurred in the vacuoles of VACs and marked changes were noted in mitochondria, plastids and the protective layer, in particular in the pit at the vessel interface. In these pits, the protective layer, the primary wall and the middle lamella were all highly modified. In contrast, treatment with eutypine induced the development of a large transfer apparatus bordering the unmodified pectocellulose wall. These results illustrate that treatment with polypeptides produced by E. lata may cause changes in mesophyll cells in leaves and VACs in canes, that resemble changes observed in naturally infected vines. Comparatively, the differences with eutypine action were stressed. Both types of toxins may co-operate in vivo to produce the degeneration observed during the disease.
Acknowledgments
We are grateful to the ‘Service Interdisciplinaire de Microscopie et d’Imagerie Scientifiques’, UFR SFA, University of Poitiers, and to JM Pérault for technical assistance. This work was supported by the Conseil Interprofessionnel du Vin de Bordeaux.
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