Register      Login
Australasian Plant Disease Notes Australasian Plant Disease Notes Society
RESEARCH ARTICLE

Candidatus Phytoplasma cynodontis’ (16SrXIV group) affecting Oplismenus burmannii (Retz.) P. Beauv. and Digitaria sanguinalis (L.) Scop. in India

G. P. Rao A C , S. Mall A and C. Marcone B
+ Author Affiliations
- Author Affiliations

A Sugarcane Research Station, Kunraghat, Gorakhpur 273 008, UP, India.

B Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, I-84084 Fisciano (Salerno), Italy.

C Corresponding author. Email: gprao_gor@rediffmail.com

Australasian Plant Disease Notes 5(1) 93-95 https://doi.org/10.1071/DN10033
Submitted: 23 August 2009  Accepted: 18 August 2010   Published: 30 August 2010

Abstract

A phytoplasma has been detected in Oplismenus burmannii (Retz.) P. Beauv. and Digitaria sanguinalis (L.) Scop. plants with symptoms of chlorotic streaks and white leaf disease in India. By sequence and phylogenetic analyses of polymerase chain reaction-amplified rDNA sequences, the detected phytoplasma was identified as ‘Candidatus Phytoplasma cynodontis’, a member of the 16SrXIV group, and the causal agent of Bermuda grass white leaf disease. This is the first report on the occurrence of ‘Ca. Phytoplasma cynodontis’ in O. burmannii and D. sanguinalis grasses.


References


Ahrens U, Seemüller E (1992) Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828–832.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Arocha Y, Singh A, Pandey M, Tripathi AN, Chandra B, Shukla SK, Singh Y, Kumar A, Srivastava RK, Zaidi NW, Arif M, Narwal S, Tewari AK, Gupta AK, Nath PD, Rabindran R, Khirbat SK, Byadgi AS, Singh G, Boa E (2009) New plant hosts for group 16SrII, ‘Candidatus Phytoplasma aurantifolia’, in India. Plant Pathology 58, 391.
Crossref | GoogleScholarGoogle Scholar | open url image1

Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiological Methods 14, 53–61.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Ghosh DK, Das AK, Shayam S, Singh SJ, Ahlawat YS, Singh S (1999) Occurrence of witches’ broom, a new phytoplasma disease of acid lime (Citrus aurantifolia) in India. Plant Disease 83, 302.
Crossref | GoogleScholarGoogle Scholar | open url image1

Gundersen DE, Lee IM (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea 35, 144–151.
CAS |
open url image1

Khan MS, Raj SK (2006) First report of molecular detection of an Aster yellows phytoplasma isolate infecting chilli (Capsicum annuum) in India. Plant Pathology 55, 822.
Crossref | GoogleScholarGoogle Scholar | open url image1

Khan MS, Raj SK, Snehi SK (2007) First report of molecular identification of ‘Candidatus Phytoplasma asteris’ affecting Sesame (Sesamum indicum) cultivation in India. Plant Pathology 89, 291–295. open url image1

Mall S (2009) Characterization of phytoplasma associated with weeds in Eastern Uttar Pradesh. Ph.D. Thesis, DDU Gorakhpur University, Gorakhpur, UP, India, p. 156.

Marcone C, Schneider B, Seemüller E (2004) ‘Candidatus Phytoplasma cynodontis’, the phytoplasma associated with Bermuda grass white leaf disease. International Journal of Systematic and Evolutionary Microbiology 54, 1077–1082.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Raj SK, Khan MS, Snehi SK, Srivastava S, Singh HB (2006) ‘Candidatus Phytoplasma asteris’ isolate associated with a little leaf disease of pigeon pea in India. Plant Pathology 55, 823.
Crossref | GoogleScholarGoogle Scholar | open url image1

Raj SK, Snehi SK, Khan MS, Kumar S (2008) ‘Candidatus Phytoplasma asteris’ (group 16SrI) associated with a witches’-broom disease of Cannabis sativa in India. Plant Pathology 57, 1173.
Crossref | GoogleScholarGoogle Scholar | open url image1

Raj SK, Snehi SK, Kumar S, Banjerji BK, Diwedi AK, Roy RK, Goel AK (2009) First report of a ‘Candidatus Phytoplasma asteris’(16SrI group) associated with colour – breaking and mall formation of floral spikes of Gladiolus in India. Plant Pathology 58, 1170.
Crossref | GoogleScholarGoogle Scholar | open url image1

Rao GP, Raj SK, Snehi SK, Mall S, Singh M, Marcone C (2007) Molecular evidence for the presence of ‘Candidatus Phytoplasma cynodontis’, the Bermuda grass white leaf agent, in India. Bulletin of Insectology 60(2), 145–146. open url image1

Rao GP, Mall S, Singh M, Marcone C (2009) First report of a ‘Candidatus Phytoplasma cynodontis’-related strain (group 16SrXIV) associated with white leaf disease of Dichanthium annulatum in India. Australasian Plant Disease Notes 4(1), 56–58.
CAS |
open url image1

Snehi SK, Khan MS, Raj SK, Mall S, Singh M, Rao GP (2008) Molecular identification of Candidatus Phytoplasma cynodontis associated with Bermuda grass white leaf disease in India. Plant Pathology 57, 770.
Crossref | GoogleScholarGoogle Scholar | open url image1

Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1