Detection of Pantoea ananatis, causal agent of leaf spot disease of maize, in Mexico
R. Pérez-y-Terrón A , M. C. Villegas B , A. Cuellar C , J. Muñoz-Rojas C , M. Castañeda-Lucio D , I. Hernández-Lucas E , R. Bustillos-Cristales C , L. Bautista-Sosa C , J. A. Munive C , R. Caicedo-Rivas A and L. E. Fuentes-Ramírez C FA Escuela de Biología, Universidad Autónoma de Puebla, Mexico.
B Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional.
C Lab. Microbiología de Suelos, Mexico.
D Lab. Bioquímica y Genética Microbiana, Instituto de Ciencias, Universidad Autónoma de Puebla, Apdo. Postal 1622, Puebla, CP 72000, México.
E Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos, CP 62250, México.
F Corresponding author. Email: lefuente@siu.buap.mx
Australasian Plant Disease Notes 4(1) 96-99 https://doi.org/10.1071/DN09041
Submitted: 27 July 2009 Accepted: 30 August 2009 Published: 21 September 2009
Abstract
Bacterial isolates from maize plants showing a leaf spot disease were identified through molecular and phenotypic traits, showing that the isolates belong to Pantoea ananatis. Maize plants inoculated with those isolates showed a pathogenic reaction. This is the first report of a disease of Mexican maize caused by P. ananatis.
Additional keywords: Enterobacteriaceae, Zea mays, phytopathogen.
Acknowledgments
This work was partially funded by projects CONACYT 000000000089747, and VIEP-BUAP, FURL-NAT08-G and BUCM-NAT09-I and by IPN. R. Pérez was granted by PROMEP. We thank Dr Alfredo G. Torres (University of Texas Medical Branch) and Dr Michael Dunn (Centro de Ciencias Genómicas, UNAM) for editing the manuscript.
Altschul SF,
Madden TL,
Schäffer AA,
Zhang J,
Zhang Z,
Miller W, Lipman DJ
(1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389–3402.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Azad HR,
Holmes GJ, Cooksey DA
(2000) A new leaf blotch disease of sudangrass caused by Pantoea ananas and Pantoea stewartii. Plant Disease 84, 973–979.
| Crossref | GoogleScholarGoogle Scholar |
Brady C,
Cleenwerck I,
Venter S,
Vancanneyt M,
Swings J, Coutinho T
(2008) Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). Systematic and Applied Microbiology 31, 447–460.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Bruton BD,
Wells JM,
Lester GE, Patterson CL
(1991) Pathogenicity and characterization of Erwinia ananas causing a postharvest disease of cantaloupe fruit. Plant Disease 75, 180–183.
Cha J-S,
Pujol C,
Ducusin AR,
Macion EA,
Hubbard C, Kado CI
(1997) Studies on Pantoea citrea, the causal agent of pink disease of pineapple. Journal of Phytopathology 145, 313–319.
| Crossref |
Cortesi P, Pizzatti C
(2007) Palea browning, a new disease of rice in Italy caused by Pantoea ananatis. Journal of Phytopathology 89, S76–S76.
Coutinho TA, Venter SN
(2009)
Pantoea ananatis: an unconventional plant pathogen. Molecular Plant Pathology 10, 325–335.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Coutinho TA,
Preisig O,
Mergaert J,
Cnockaert MC,
Riedel KH,
Swings J, Wingfield MJ
(2002) Bacterial blight and dieback of Eucalyptus species, hybrids, and clones in South Africa. Plant Disease 86, 20–25.
| Crossref | GoogleScholarGoogle Scholar |
Dauga C
(2002) Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies. International Journal of Systematic and Evolutionary Microbiology 52, 531–547.
|
CAS |
PubMed |
Fucikovsky L, Aranda S
(2006)
Pantoea ananas a new pathogen of agave in Mexico. Phytopathology 96, S37–S37.
Fuentes-Ramírez LE,
Caballero-Mellado J,
Sepúlveda J, Martínez-Romero E
(1999) Colonization of sugar cane by Acetobacter diazotrophicus is inhibited by high N-fertilization. FEMS Microbiology Ecology 29, 117–128.
Goszczynska T,
Botha WJ,
Venter SN, Coutinho TA
(2007) Isolation and identification of the causal agent of brown stalk rot, a new disease of maize in South Africa. Plant Disease 91, 711–718.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Lakso JU, Starr MP
(1970) Comparative injuriousness to plants of Erwinia spp. and other enterobacteria from plants and animals. Journal of Applied Bacteriology 33, 692–707.
|
CAS |
PubMed |
Mergaert J,
Verdonck L, Kersters K
(1993) Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov. International Journal of Systematic and Evolutionary Microbiology 43, 162–173.
| Crossref |
Paccola-Meirelles LD,
Ferreira AS,
Meirelles WF,
Marriel IE, Casela CR
(2001) Detection of a bacterium associated with a leaf spot disease of maize in Brazil. Journal of Phytopathology 149, 275–279.
| Crossref | GoogleScholarGoogle Scholar |
Sergeeva E,
Hirkala DLM, Nelson LM
(2007) Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates. Plant and Soil 297, 1–13.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Weisburg WG,
Barns SM,
Pelletier DA, Lane DJ
(1991) 16S ribosomal amplification for phylogenetic study. Journal of Bacteriology 173, 697–703.
|
CAS |
PubMed |