Toxicity of pure compounds isolated from Tagetes minuta oil to Meloidogyne incognita

O. K. Adekunle; Ruchi Acharya; Bikram Singh

doi:10.1071/DN07042

RESEARCH ARTICLE

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Toxicity of pure compounds isolated from Tagetes minuta oil to Meloidogyne incognita

O. K. Adekunle ^A ^C ^D , Ruchi Acharya ^B and Bikram Singh ^B

+ Author Affiliations

- Author Affiliations

^A Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India.

^B Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, HP 176 061, India.

^C Present address: Plant Nematology Laboratory, Department of Plant Science, Obafemi Awolowo University, Ile-Ife, Nigeria.

^D Corresponding author. Email: kolaade2002@yahoo.co.uk

Australasian Plant Disease Notes 2(1) 101-104 https://doi.org/10.1071/DN07042
Submitted: 9 October 2006 Accepted: 21 June 2007 Published: 3 July 2007

Abstract

Essential oils are natural volatile substances found in a variety of plants. The toxicity of z-β-ocimene and dihydrotagetone (isolated from the oil of Tagetes minuta) to eggs and juveniles of Meloidogyne incognita was investigated in vitro. Tagetes minuta oil at 4%, 3%, 2% and 1% was strongly toxic to eggs and juveniles of M. incognita. Further studies revealed that dihydrotagetone and z-β-ocimene isolated from the oil showed strong nematicidal activity against M. incognita, with dihydrotagetone showing a higher level of toxicity than z-β-ocimene. The results of this study suggest that dihydrotagetone and z-β-ocimene isolated from T. minuta oil are potential sources of botanicals for control of the root-knot nematode M. incognita.

Additional keywords: root-knot nematode, characterisation.

Acknowledgements

Dr O. K. Adekunle acknowledges award of a postdoctoral fellowship from Third World Academy of Sciences (TWAS), Trieste, Italy. Authors are thankful to the Director, Institute of Himalayan Bioresource Technology for encouragement and providing necessary research facilities. Professor Akhtar Hasseb of the Institute of Agriculture, Aligarh Muslim University, India is gratefully acknowledged for providing cultures of Meloidogyne incognita. Drs Sandeep Manuja and Raja Ram, IHBT, Palampur are thanked for running statistical analysis. IHBT Comm. No. is 0503.

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