Natural occurrence of Cucumber mosaic virus on lemongrass (Cymbopogon citratus), a new record
S. K. Raj A C , S. Kumar A , D. Pratap A , R. Vishnoi A , S. Choudhari A and S. Chandra BA Molecular Virology Laboratory, National Botanical Research Institute, Lucknow 226 001, India.
B Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine, Jammu-Tawi 180 001, India.
C Corresponding author. Email: skraj2@rediffmail.com
Australasian Plant Disease Notes 2(1) 95-96 https://doi.org/10.1071/DN07039
Submitted: 10 May 2007 Accepted: 12 June 2007 Published: 22 June 2007
Abstract
The natural occurrence of Cucumber mosaic virus (CMV) on Cymbopogon citratus was detected by enzyme linked immunosorbent assay using antiserum raised against CMV and reverse transcription polymerase chain reaction using specific primers for the coat protein. Sequence analysis of the PCR product showed 93–97% identities and a close phylogenetic relationship with CMV isolates of subgroup Ib.
Acknowledgements
The authors express gratitude to the Directors NBRI, Lucknow and IIIM, Jammu-Tawi for permission and CSIR, New Delhi for the fellowships to S. Kumar and D. Pratap.
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