The first report of transmission of a phytoplasma associated with sesame phyllody by Orosius albicinctus in Iran
S. A. Esmailzadeh-Hosseini A D , A. Mirzaie B , A. Jafari-Nodooshan A and H. Rahimian CA Yazd Agricultural and Natural Resources Research Center, Plant Pests and Diseases Research Department, Yazd, Iran.
B Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
C Plant Protection Department, Mazandaran University, Sari, Iran.
D Corresponding author. Email: saesmailzadeh@yahoo.com
Australasian Plant Disease Notes 2(1) 33-34 https://doi.org/10.1071/DN07016
Submitted: 31 January 2006 Accepted: 12 March 2007 Published: 3 April 2007
Abstract
During 2003–05, a survey for phytoplasmas was carried out in sesame fields in Yazd province (Iran). The leafhopper Orosius albicinctus was collected and found to contain a phytoplasma. Transmission tests were performed under greenhouse conditions. The indicator plants were alfalfa, rapeseed, garden beet, sesame, radish, periwinkle and garden cress. A nested polymerase chain reaction using phytoplasma universal primers was used to detect phytoplasmas in indicator plants and leafhoppers. Amplification of a phytoplasma-characteristic 1.2-kb 16 S rDNA fragment confirmed that sesame, garden cress and garden beet plants were infected by the phytoplasma. On the basis of RFLP analysis of the amplified DNA fragments using five endonucleases, the detected phytoplasma belonged to the 16SrII group (peanut witches’ broom phytoplasma). This is the first report of transmission of a phytoplasma associated with sesame phyllody by O. albicinctus in Iran.
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