A potyvirus in Cymbidium spp. in northern India

M. K. Singh; A. R. Sherpa; V. Hallan; A. A. Zaidi

doi:10.1071/DN07005

RESEARCH ARTICLE

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A potyvirus in Cymbidium spp. in northern India

M. K. Singh ^A , A. R. Sherpa ^A , V. Hallan ^A and A. A. Zaidi ^A ^B

+ Author Affiliations

- Author Affiliations

^A Plant Virology Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur-176 061, Himachal Pradesh, India.

^B Corresponding author. Email: zaidi_aijaz@yahoo.com

Australasian Plant Disease Notes 2(1) 11-13 https://doi.org/10.1071/DN07005
Submitted: 18 October 2006 Accepted: 20 December 2006 Published: 1 February 2007

Abstract

A potyvirus was isolated from diseased Cymbidium pendulum and C. tigrinum epiphytic orchids collected from Sikkim in northern India. ELISA, RT-PCR and Northern blot analysis revealed the association of potyvirus infection with disease symptoms. The sequencing of a RT-PCR-amplified amplicon using potyvirus group-specific primers showed that this virus is distinct but most closely related to Calanthe mild mosaic virus. This paper reports the occurrence of potyvirus in wild orchids especially in Cymbidium spp.

Acknowledgements

We are thankful to the Director, IHBT, Palampur, for necessary facilitues and to DBT, Government of India, for financial support (Project no. BT/PR/1790/AGR/08/126/99). IHBT communication Number: 0158.

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