Simulated Stern-Volmer Plots for 1-1 Ground-State Complexation

Abstract

Stern-Volmer plots are simulated for the case of static quenching due to 1:1 ground-state complexation of quencher and fluorophore in solution, and compared with previously obtained experimental data. For cases where the association constant K_C > 10⁵ dm³ mol^-1, upward curvatures of Stern- Volmer plots are expected. When K_C is between 10³ and 10⁵ dm³ mol-¹ linearity is obtained, while for K_C < 10³ dm³ mol^-1 upward curvatures are again predicted. The significance of these results to fluorescence quenching experiments in general is discussed.