Organophosphorus compounds. VIII. Diphenylphosphinothioic acid derivatives as reagents for proteins

Abstract

Esters and other derivatives of diphenylphosphinothioic acids are examined as potential agents for the radioactive labelling of immunoglobulins and other proteins. Syntheses, model reactions with amines, and mass spectra of the organo-phosphorus compounds are discussed. The labelling of immunoglobulins by reaction with [³²P]diphenylphosphinothioic chloride, with [³²P]diphenylphosphinothioic isothiocyanate, and with [³²P]diphenylphosphinodithioic acid in the presence of condensing agents such as dimethylcyanamide and carbodiimides is reported.