Water-soluble constituents of fruit. II. The separation of acids on anion-exchange resins: the isolation of l-auinic acid from Apricots

Abstract

Displacement chromatography on columns of strongly basic anion-exchange resins has been used to separate, isolate, and purify some water-soluble organic acids. The order of emergence of 27 acids from these columns is recorded. Using this method pure l-quinic, succinic, L-malic, and citric acids were isolated from the flesh of the apricot fruit. The method was also used for the purification of hydroxy-acids. The detection of acids on paper chromatograms with a silver nitrate reagent is described ; silver nitrate-sodium hydroxide was used to detect acids having a vic.-glycol or α-keto group. The R_F values for 30 acids in two solvent systems are recorded.